Dietary nanoparticles alter the composition and function of the gut microbiota in mice at dose levels relevant for human exposure.
Administration, Oral
Animals
Bacteria
/ drug effects
Dietary Exposure
/ adverse effects
Fatty Acids, Volatile
/ metabolism
Gastrointestinal Microbiome
/ drug effects
Interleukin-6
/ metabolism
Male
Metal Nanoparticles
/ administration & dosage
Mice, Inbred C57BL
Silicon Dioxide
/ administration & dosage
Silver
/ administration & dosage
Titanium
/ administration & dosage
Triglycerides
/ metabolism
Ag NP
Food
Lipid metabolism
Long-term effects
SiO(2) NP
TiO(2) NP
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
27
04
2021
revised:
02
06
2021
accepted:
12
06
2021
pubmed:
22
6
2021
medline:
3
11
2021
entrez:
21
6
2021
Statut:
ppublish
Résumé
Nanotechnologies provide new opportunities for improving the safety, quality, shelf life, flavor and appearance of foods. The most common nanoparticles (NPs) in human diet are silver metal, mainly present in food packaging and appliances, and silicon and titanium dioxides used as additives. The rapid development and commercialization of consumer products containing these engineered NPs is, however, not well supported by appropriate toxicological studies and risk assessment. Local and systemic toxicity and/or disruption of the gut microbiota (GM) have already been observed after oral administration of NPs in experimental animals, but results are not consistent and doses used were often much higher than the estimated human intakes. In view of the strong evidence linking alterations of the GM to cardiometabolic (CM) diseases, we hypothesized that dietary NPs might disturb this GM-CM axis. We exposed male C57BL/6JRj mice (n = 13 per dose group) to dietary NPs mixed in food pellets at doses relevant for human exposure: Ag (0, 4, 40 or 400 μg/kg pellet), SiO No overt toxicity was recorded. The GM β-diversity was dose-dependently disrupted by the three NPs, and the bacterial short chain fatty acids (SCFAs) were dose-dependently reduced after the administration of SiO We found that long-term oral exposure to dietary NPs at doses relevant for estimated human intakes disrupts the GM composition and function. These modifications did not appear associated with atheromatous or deleterious metabolic outcomes.
Sections du résumé
BACKGROUND
BACKGROUND
Nanotechnologies provide new opportunities for improving the safety, quality, shelf life, flavor and appearance of foods. The most common nanoparticles (NPs) in human diet are silver metal, mainly present in food packaging and appliances, and silicon and titanium dioxides used as additives. The rapid development and commercialization of consumer products containing these engineered NPs is, however, not well supported by appropriate toxicological studies and risk assessment. Local and systemic toxicity and/or disruption of the gut microbiota (GM) have already been observed after oral administration of NPs in experimental animals, but results are not consistent and doses used were often much higher than the estimated human intakes. In view of the strong evidence linking alterations of the GM to cardiometabolic (CM) diseases, we hypothesized that dietary NPs might disturb this GM-CM axis.
MATERIALS AND METHODS
METHODS
We exposed male C57BL/6JRj mice (n = 13 per dose group) to dietary NPs mixed in food pellets at doses relevant for human exposure: Ag (0, 4, 40 or 400 μg/kg pellet), SiO
RESULTS
RESULTS
No overt toxicity was recorded. The GM β-diversity was dose-dependently disrupted by the three NPs, and the bacterial short chain fatty acids (SCFAs) were dose-dependently reduced after the administration of SiO
CONCLUSION
CONCLUSIONS
We found that long-term oral exposure to dietary NPs at doses relevant for estimated human intakes disrupts the GM composition and function. These modifications did not appear associated with atheromatous or deleterious metabolic outcomes.
Identifiants
pubmed: 34153347
pii: S0278-6915(21)00385-9
doi: 10.1016/j.fct.2021.112352
pii:
doi:
Substances chimiques
Fatty Acids, Volatile
0
Interleukin-6
0
Triglycerides
0
interleukin-6, mouse
0
titanium dioxide
15FIX9V2JP
Silver
3M4G523W1G
Silicon Dioxide
7631-86-9
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112352Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.