CALM supports clathrin-coated vesicle completion upon membrane tension increase.
clathrin adaptors
lattice light-sheet microscopy
membrane tension
membrane traffic
quantitative microscopy
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
22 06 2021
22 06 2021
Historique:
entrez:
22
6
2021
pubmed:
23
6
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
The most represented components of clathrin-coated vesicles (CCVs) are clathrin triskelia and the adaptors clathrin assembly lymphoid myeloid leukemia protein (CALM) and the heterotetrameric complex AP2. Investigation of the dynamics of AP180-amino-terminal-homology (ANTH) recruitment during CCV formation has been hampered by CALM toxicity upon overexpression. We used knock-in gene editing to express a C-terminal-attached fluorescent version of CALM, while preserving its endogenous expression levels, and cutting-edge live-cell microscopy approaches to study CALM recruitment at forming CCVs. Our results demonstrate that CALM promotes vesicle completion upon membrane tension increase as a function of the amount of this adaptor present. Since the expression of adaptors, including CALM, differs among cells, our data support a model in which the efficiency of clathrin-mediated endocytosis is tissue specific and explain why CALM is essential during embryogenesis and red blood cell development.
Identifiants
pubmed: 34155137
pii: 2010438118
doi: 10.1073/pnas.2010438118
pmc: PMC8237669
pii:
doi:
Substances chimiques
Adaptor Protein Complex 2
0
Monomeric Clathrin Assembly Proteins
0
PICALM protein, human
0
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : P30 CA016058
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127526
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Nat Cell Biol. 2011 Mar;13(3):331-7
pubmed: 21297641
ACS Chem Biol. 2019 May 17;14(5):882-892
pubmed: 30978004
Mol Biol Cell. 2017 Nov 15;28(24):3480-3488
pubmed: 28904210
Cancer Discov. 2012 May;2(5):401-4
pubmed: 22588877
PLoS One. 2014 Mar 11;9(3):e91242
pubmed: 24618820
Commun Biol. 2020 Dec 8;3(1):743
pubmed: 33293652
Science. 2001 Feb 9;291(5506):1051-5
pubmed: 11161218
J Cell Biol. 2018 Sep 3;217(9):3301-3311
pubmed: 29980624
Traffic. 2020 Sep;21(9):603-616
pubmed: 32657003
Nat Cell Biol. 2012 Sep;14(9):944-9
pubmed: 22902598
Science. 2019 Jan 18;363(6424):
pubmed: 30655415
Philos Trans R Soc Lond B Biol Sci. 1998 Dec 29;353(1378):1969-81
pubmed: 10098215
Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):517-33
pubmed: 21779028
Mol Biol Cell. 2010 Sep 1;21(17):3070-9
pubmed: 20631253
Cold Spring Harb Perspect Biol. 2014 Aug 28;6(12):a017038
pubmed: 25167858
J Cell Biol. 1999 Feb 8;144(3):497-506
pubmed: 9971744
Mol Biol Cell. 2009 Jul;20(14):3251-60
pubmed: 19458185
Cell. 2012 Aug 3;150(3):495-507
pubmed: 22863004
ACS Chem Biol. 2008 Jun 20;3(6):373-82
pubmed: 18533659
Science. 2014 Oct 24;346(6208):1257998
pubmed: 25342811
Methods Mol Biol. 2018;1847:197-216
pubmed: 30129019
Mol Cell. 2018 Sep 20;71(6):1079-1091.e9
pubmed: 30146318
Mol Biol Cell. 2014 Nov 5;25(22):3595-609
pubmed: 25232009
Br J Cancer. 1999 Dec;81(8):1328-34
pubmed: 10604729
Elife. 2020 Apr 30;9:
pubmed: 32352376
Nat Methods. 2015 Mar;12(3):244-50, 3 p following 250
pubmed: 25599551
Annu Rev Biochem. 2018 Jun 20;87:871-896
pubmed: 29661000
PLoS Biol. 2009 Mar 17;7(3):e57
pubmed: 19296720
PLoS Biol. 2011 Mar;9(3):e1000604
pubmed: 21445324
BMC Neurosci. 2016 Jul 18;17(1):50
pubmed: 27430330
Dev Cell. 2015 Apr 20;33(2):163-75
pubmed: 25898166
Nature. 1995 Feb 23;373(6516):663-4
pubmed: 7854443
Nat Methods. 2007 Apr;4(4):319-21
pubmed: 17369835
Gene. 1983 Dec;26(1):101-6
pubmed: 6323249
Mol Biol Cell. 1999 Aug;10(8):2687-702
pubmed: 10436022
Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3833-8
pubmed: 15007177
J Cell Biol. 2016 Aug 1;214(3):347-58
pubmed: 27458134
PLoS One. 2012;7(2):e31854
pubmed: 22363754
Protein Sci. 2013 Feb;22(2):153-67
pubmed: 23225024
J Cell Sci. 2017 Nov 1;130(21):3631-3636
pubmed: 28923837
BMC Biotechnol. 2008 Dec 04;8:91
pubmed: 19055817
Cell Rep. 2012 Nov 29;2(5):1111-9
pubmed: 23103167
Nat Genet. 2013 Jun;45(6):580-5
pubmed: 23715323
Mol Biol Cell. 2016 Nov 7;27(22):3418-3435
pubmed: 27535432
Membranes (Basel). 2014 Jul 31;4(3):388-413
pubmed: 25090048
Nat Cell Biol. 2011 Aug 14;13(9):1124-31
pubmed: 21841790
Methods Enzymol. 2012;505:59-80
pubmed: 22289448
J Cell Biol. 2020 Sep 7;219(9):
pubmed: 32770195
Cell. 2004 Sep 3;118(5):591-605
pubmed: 15339664
Cell. 2011 Nov 23;147(5):1118-31
pubmed: 22118466
Bioinformatics. 2019 Dec 15;35(24):5393-5395
pubmed: 31240306
Haematologica. 2015 Apr;100(4):439-51
pubmed: 25552701
J Cell Sci. 2011 May 15;124(Pt 10):1613-22
pubmed: 21536832
Nat Methods. 2018 Jan;15(1):47-51
pubmed: 29320486
Biophys J. 2018 Apr 24;114(8):2014-2023
pubmed: 29694877
Cold Spring Harb Symp Quant Biol. 1995;60:567-71
pubmed: 8824429
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8360-5
pubmed: 12832620
Genome Res. 2014 Jan;24(1):132-41
pubmed: 24253446
Cold Spring Harb Perspect Biol. 2014 May 01;6(5):a016725
pubmed: 24789820
PLoS Biol. 2011 Mar;9(3):e1001037
pubmed: 21445329
Mol Biol Cell. 2020 Aug 15;31(18):2035-2047
pubmed: 32579424
J Cell Sci. 2001 Jan;114(Pt 2):353-65
pubmed: 11148137
J Cell Biol. 2014 Jun 9;205(5):721-35
pubmed: 24891602
Sci Signal. 2013 Apr 02;6(269):pl1
pubmed: 23550210
Dev Cell. 2013 Aug 12;26(3):279-91
pubmed: 23891661
Blood. 2004 Nov 1;104(9):2661-6
pubmed: 15238415
Nat Commun. 2015 Feb 19;6:6249
pubmed: 25695735
Nat Protoc. 2013 Nov;8(11):2281-2308
pubmed: 24157548
Curr Alzheimer Res. 2019;16(13):1196-1205
pubmed: 31385771
Traffic. 2005 Dec;6(12):1225-34
pubmed: 16262731
J Cell Biol. 2012 Apr 2;197(1):141-60
pubmed: 22472443
J Biol Chem. 2004 Apr 16;279(16):16657-61
pubmed: 14985334
Haematologica. 2006 Feb;91(2):184-91
pubmed: 16461302
Mol Cell Proteomics. 2012 Mar;11(3):M111.011429
pubmed: 21937730
Nat Commun. 2018 Jan 29;9(1):419
pubmed: 29379015
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
J Biol Chem. 2012 Jun 15;287(25):21279-89
pubmed: 22539346
Nat Methods. 2008 Aug;5(8):695-702
pubmed: 18641657
Curr Opin Chem Biol. 2017 Aug;39:32-38
pubmed: 28544971
Genome Med. 2009 Jun 16;1(6):62
pubmed: 19566914