Bi/tri-specific antibodies (HN-Fc-CD16 and HN-Fc-IL-15-CD16) cross-linking natural killer (NK)-CD16 and Newcastle Disease Virus (NDV)-HN, enhanced NK activation for cancer immunotherapy.
Antibodies, Bispecific
/ biosynthesis
Antineoplastic Agents, Immunological
/ chemistry
Binding Sites
Cytotoxicity Tests, Immunologic
HEK293 Cells
HN Protein
/ immunology
HeLa Cells
Humans
Immunoglobulin Fc Fragments
/ immunology
Immunotherapy
/ methods
Interferon-gamma
/ metabolism
Killer Cells, Natural
/ immunology
Ligands
Models, Molecular
Neoplasms
/ immunology
Newcastle disease virus
/ immunology
Receptors, IgG
/ immunology
Bispecific antibody
Immunotherapy
Mammalian expression system
Natural Killer cell (NK cell)
Newcastle Disease Virus (NDV)
Trispecific antibody
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
23
11
2020
revised:
22
04
2021
accepted:
04
05
2021
entrez:
24
6
2021
pubmed:
25
6
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Cancer/tumor cells infected with the "avian paramyxovirus Newcastle Disease Virus (TC-NDV)" express the viral hemagglutinin-neuraminidase (HN) on the cell surface that is used as both the danger signal and anchor for bi/tri-specific antibodies (bs/tsAbs).We constructed a bs-Ab (HN-Fc-CD16) that bindsto HN and natural killer (NK)-CD16 receptor (FcgRIII)and a ts-Ab (HN-Fc-IL15-CD16) harbouring NK-activating cytokine "IL-15" within the bs-Ab.In silicoand computational predictions indicated proper exposure of both Abs in bs/tsAbs.Properbinding of thebi/tsAbstoHN on surface of TC-NDVandCD16
Identifiants
pubmed: 34162140
pii: S1567-5769(21)00398-2
doi: 10.1016/j.intimp.2021.107762
pii:
doi:
Substances chimiques
Antibodies, Bispecific
0
Antineoplastic Agents, Immunological
0
HN Protein
0
Immunoglobulin Fc Fragments
0
Ligands
0
Receptors, IgG
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107762Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.