Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering.

AFM, atomic force microscopy APTES, (3-aminopropyl) triethoxysilane ATR, attenuated total reflection DPBS, Dulbecco's phosphate-buffered saline EthHD1, ethidium homodimer 1 FT-IR, Fourier-transform infrared (spectroscopy) IPTG, isopropyl-β-D-thiogalactoside MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis eADF4, Engineered Araneus diadematus fibroin 4 hiPSC, human-induced pluripotent stem cell

Journal

Materials today. Bio
ISSN: 2590-0064
Titre abrégé: Mater Today Bio
Pays: England
ID NLM: 101757228

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 15 03 2021
revised: 01 05 2021
accepted: 08 05 2021
entrez: 25 6 2021
pubmed: 26 6 2021
medline: 26 6 2021
Statut: epublish

Résumé

Materials made of recombinant spider silk proteins are promising candidates for cardiac tissue engineering, and their suitability has so far been investigated utilizing primary rat cardiomyocytes. Herein, we expanded the tool box of available spider silk variants and demonstrated for the first time that human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes attach, contract, and respond to pharmacological treatment using phenylephrine and verapamil on explicit spider silk films. The hiPSC-cardiomyocytes contracted for at least 14 days on films made of positively charged engineered

Identifiants

DOI: 10.1016/j.mtbio.2021.100114 PMID: 34169268 PMC: PMC8209670
pubmed: 34169268
doi: 10.1016/j.mtbio.2021.100114
pii: S2590-0064(21)00022-3
pmc: PMC8209670
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100114

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Prof. Scheibel is co-founder and shareholder of AMSilk GmbH.

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Auteurs

T U Esser (TU)

Experimental Renal and Cardiovascular Research, Department of Nephropathology, Institute of Pathology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, 91054, Germany.

V T Trossmann (VT)

Lehrstuhl Biomaterialien, Prof.-Rüdiger-Bormann Straße 1, Bayreuth, 95447, Germany.

S Lentz (S)

Lehrstuhl Biomaterialien, Prof.-Rüdiger-Bormann Straße 1, Bayreuth, 95447, Germany.

F B Engel (FB)

Experimental Renal and Cardiovascular Research, Department of Nephropathology, Institute of Pathology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, 91054, Germany.
MURCE, Muscle Research Center Erlangen, Erlangen, Germany.

T Scheibel (T)

Lehrstuhl Biomaterialien, Prof.-Rüdiger-Bormann Straße 1, Bayreuth, 95447, Germany.
Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayerisches Polymerinstitut (BPI), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Universitätsstraße 30, Universität Bayreuth, Bayreuth, D-95447, Germany.

Classifications MeSH