Successful retrieval of human papillomavirus DNA after a 4.5 year storage on FTA elute cards.
Biobanking
DNA preservation
FTA
HPV
Papillomavirus
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
02
10
2020
revised:
19
04
2021
accepted:
23
06
2021
pubmed:
28
6
2021
medline:
25
11
2021
entrez:
27
6
2021
Statut:
ppublish
Résumé
Efficient primary (vaccination) and secondary (screening) prevention strategies have the potential to eliminate cervical cancer worldwide. In this context, surveillance of HPV infections remains mandatory to assess the efficacy and the impact of screening and vaccination policies. Therefore there is a need to safely store cervical samples to conduct long-term studies in vaccinated and non-vaccinated subjects. Up-dated data on cervical specimen preservation on FTA® cards indicate that HPV DNA can be safely retrieved after 54 months of storage. A concordance of 97 % was achieved between HPV genotypes detected in initial cervical samples and on FTA® cards 4.5 years later. Even if a drop in HPV viral loads was observed in some cases at 4.5 years, using FTA® cards for safe and long-term storage of cervical samples represents an interesting option.
Identifiants
pubmed: 34175346
pii: S0166-0934(21)00157-9
doi: 10.1016/j.jviromet.2021.114218
pii:
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114218Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.