Detectable SARS-CoV-2 RNAemia in Critically Ill Patients, but Not in Mild and Asymptomatic Infections.

Blood donation Blood safety Plasma Virus safety

Journal

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie
ISSN: 1660-3796
Titre abrégé: Transfus Med Hemother
Pays: Switzerland
ID NLM: 101176417

Informations de publication

Date de publication:
May 2021
Historique:
received: 27 10 2020
accepted: 05 03 2021
entrez: 28 6 2021
pubmed: 29 6 2021
medline: 29 6 2021
Statut: ppublish

Résumé

The SARS-CoV-2 pandemic has challenged many of our current routine practices in the treatment and care of patients. Given the critical importance of blood donation and transfusion we analyzed 92 blood samples of individuals infected with SARS-CoV-2 stratified by symptoms. We therefore tested blood samples for SARS-CoV-2 via RT-PCR targeting the E gene. In addition, we tested each blood sample for anti-SARS-CoV-2 IgG antibodies via ELISA and performed plaque reduction neutralization tests. SARS-CoV-2 RNA was absent in the blood of mild to asymptomatic patients (57 individuals) and only detectable in individuals with severe COVID-19 who were admitted to the intensive care unit (35 individuals) ( Our observations support the hypothesis that there are no potential hazards from blood or plasma transfusion of SARS-CoV-2-positive individuals with mild flu-like symptoms and more importantly of asymptomatic individuals.

Sections du résumé

BACKGROUND BACKGROUND
The SARS-CoV-2 pandemic has challenged many of our current routine practices in the treatment and care of patients. Given the critical importance of blood donation and transfusion we analyzed 92 blood samples of individuals infected with SARS-CoV-2 stratified by symptoms.
STUDY DESIGN AND METHODS METHODS
We therefore tested blood samples for SARS-CoV-2 via RT-PCR targeting the E gene. In addition, we tested each blood sample for anti-SARS-CoV-2 IgG antibodies via ELISA and performed plaque reduction neutralization tests.
RESULTS RESULTS
SARS-CoV-2 RNA was absent in the blood of mild to asymptomatic patients (57 individuals) and only detectable in individuals with severe COVID-19 who were admitted to the intensive care unit (35 individuals) (
CONCLUSION CONCLUSIONS
Our observations support the hypothesis that there are no potential hazards from blood or plasma transfusion of SARS-CoV-2-positive individuals with mild flu-like symptoms and more importantly of asymptomatic individuals.

Identifiants

pubmed: 34177419
doi: 10.1159/000515841
pii: tmh-0048-0154
pmc: PMC8216035
doi:

Types de publication

Journal Article

Langues

eng

Pagination

154-160

Informations de copyright

Copyright © 2021 by S. Karger AG, Basel.

Déclaration de conflit d'intérêts

The authors have declared no competing interest.

Références

PLoS One. 2021 Jan 28;16(1):e0245845
pubmed: 33508030
J Virus Erad. 2019 Jan 1;5(1):3-9
pubmed: 30800420
Emerg Infect Dis. 2020 Jul;26(7):1631-1633
pubmed: 32243255
Pathog Immun. 2020 May 16;5(1):117-120
pubmed: 32582871
Transfusion. 2020 Jun;60(6):1119-1122
pubmed: 32361996
Environ Res. 2020 Sep;188:109819
pubmed: 32569870
Emerg Microbes Infect. 2020 Dec;9(1):940-948
pubmed: 32357808
J Korean Med Sci. 2020 Feb 24;35(7):e86
pubmed: 32080991
J Allergy Clin Immunol. 2002 Aug;110(2 Suppl):S21-6
pubmed: 12170239
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
Nat Commun. 2020 Nov 17;11(1):5829
pubmed: 33203887
J Artif Organs. 2020 Dec;23(4):397-400
pubmed: 32556649
Cold Spring Harb Perspect Med. 2012 Apr;2(4):a007161
pubmed: 22474613
J Clin Virol. 2011 Sep;52(1):4-10
pubmed: 21680238
Ann Intern Med. 2021 Jan;174(1):69-79
pubmed: 32941052
JAMA. 2020 Jun 16;323(23):2425-2427
pubmed: 32421144
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Clin Vaccine Immunol. 2012 Jul;19(7):1012-8
pubmed: 22573737
Sci Immunol. 2020 Dec 7;5(54):
pubmed: 33288645
Infect Control Hosp Epidemiol. 2020 Jul;41(7):857-859
pubmed: 32268929
BMC Med. 2020 Oct 22;18(1):332
pubmed: 33087179

Auteurs

Enrico Richter (E)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Doaa Al Arashi (D)

Department for Internal Medicine, City Hospital Heinsberg, Heinsberg, Germany.

Bianca Schulte (B)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Christian Bode (C)

Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany.

Benjamin Marx (B)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Souhaib Aldabbagh (S)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Celina Schlüter (C)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Beate Mareike Kümmerer (BM)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Johannes Oldenburg (J)

Department of Experimental Hematology and Transfusion Medicine, University Hospital, University of Bonn, Bonn, Germany.

Markus B Funk (MB)

Department Safety of Drugs and Medical Devices, Paul-Ehrlich-Institut, Langen, Germany.

Christian Putensen (C)

Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany.

Ricarda Maria Schmithausen (RM)

Institute for Hygiene and Public Health, University Hospital, University of Bonn, Bonn, Germany.

Gunther Hartmann (G)

Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Anna Eis-Hübinger (A)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Hendrik Streeck (H)

Institute of Virology, University Hospital, University of Bonn, and German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.

Classifications MeSH