The moonlighting protein fructose 1,6-bisphosphate aldolase as a potential vaccine candidate against Photobacterium damselae subsp. piscicida in Asian sea bass (Lates calcarifer).
Animals
Antibodies, Bacterial
/ blood
Aquaculture
Bacterial Load
/ immunology
Bacterial Proteins
/ genetics
Bacterial Vaccines
/ administration & dosage
Fish Diseases
/ microbiology
Fructose-Bisphosphate Aldolase
/ genetics
Gram-Negative Bacterial Infections
/ microbiology
Muramidase
/ blood
Perciformes
/ immunology
Photobacterium
/ enzymology
Recombinant Proteins
/ genetics
Spleen
/ immunology
Vaccination
/ veterinary
Vaccine Efficacy
Vaccines, Subunit
/ administration & dosage
Moonlighting protein
Photobacterium damselae subsp. piscicida
Recombinant proteins
Vaccination
Journal
Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
22
03
2021
revised:
25
06
2021
accepted:
25
06
2021
pubmed:
30
6
2021
medline:
25
3
2022
entrez:
29
6
2021
Statut:
ppublish
Résumé
Vaccination is the most effective, safe, and environmentally friendly method to prevent the outbreak of Photobacterium damselae subsp. piscicida (Phdp), a dangerous pathogen in aquaculture worldwide. Here, recombinant proteins of catalase, superoxide dismutase, isocitrate dehydrogenase, fructose 1,6-bisphosphate aldolase (Fba), and a mixture of all four proteins were investigated for their immunoprotective effects against photobacteriosis in Asian sea bass (Lates calcarifer). After immunization, experimental fish showed an increase in specific antibody levels and lysozyme activities, especially the Fba group. After a lethal challenge with Phdp strain AOD105021, the Fba group achieved the highest relative percentage of survival rate (70.21%) and a significantly lower bacterial load in the spleens than other groups 3 days after infection. The results suggest that Fba is a good candidate for subunit vaccine development against photobacteriosis in fish.
Identifiants
pubmed: 34186149
pii: S0145-305X(21)00195-6
doi: 10.1016/j.dci.2021.104187
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Proteins
0
Bacterial Vaccines
0
Recombinant Proteins
0
Vaccines, Subunit
0
Muramidase
EC 3.2.1.17
Fructose-Bisphosphate Aldolase
EC 4.1.2.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104187Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.