A DNA-based method for distinction of fly artifacts from human bloodstains.
Bloodstain pattern analysis
COI sequence
Fly artifacts
Forensic genetics
Species identification
mtDNA
Journal
International journal of legal medicine
ISSN: 1437-1596
Titre abrégé: Int J Legal Med
Pays: Germany
ID NLM: 9101456
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
09
04
2021
accepted:
16
06
2021
pubmed:
1
7
2021
medline:
3
3
2022
entrez:
30
6
2021
Statut:
ppublish
Résumé
Fly artifacts resulting from insect activity could act as confounding factors on a crime scene and interfere with bloodstain pattern analysis interpretation. Several techniques have been proposed to distinguish fly artifacts from human bloodstains based on morphological approach and immunological assay, but a DNA-based method has not been developed so far. Even if in forensic genetic investigations the detection of human DNA is generally the primary goal, fly artifacts can provide useful information on the dynamics of crime events. The present study provides a molecular method to detect fly DNA from artifacts deposited by Calliphora vomitoria after feeding on human blood through the analysis of the mitochondrial cytochrome oxidase gene subunit I (COI). Fly artifacts originated from digestive process and of different morphology spanning from red and brownish/light brown, circular and elliptical stains to artifacts with sperm-like tail or a tear-shaped body were collected. The COI amplification was successfully obtained in 94% of fly artifact samples. The method showed high sensitivity and reproducibility, and no human DNA contamination was observed, offering specificity for use in confirmatory test. This molecular approach permits the distinction of fly artifacts from genuine bloodstains and the identification of fly's species through the COI region sequencing by protocols usually applied in forensic genetic laboratories.
Identifiants
pubmed: 34191097
doi: 10.1007/s00414-021-02643-7
pii: 10.1007/s00414-021-02643-7
pmc: PMC8523429
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2155-2161Informations de copyright
© 2021. The Author(s).
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