High-Resolution Human Kidney Molecular Histology by Imaging Mass Spectrometry of Lipids.


Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
13 07 2021
Historique:
pubmed: 1 7 2021
medline: 22 7 2021
entrez: 30 6 2021
Statut: ppublish

Résumé

For many years, traditional histology has been the gold standard for the diagnosis of many diseases. However, alternative and powerful techniques have appeared in recent years that complement the information extracted from a tissue section. One of the most promising techniques is imaging mass spectrometry applied to lipidomics. Here, we demonstrate the capabilities of this technique to highlight the architectural features of the human kidney at a spatial resolution of 10 μm. Our data demonstrate that up to seven different segments of the nephron and the interstitial tissue can be readily identified in the sections according to their characteristic lipid fingerprints and that such fingerprints are maintained among different individuals (

Identifiants

pubmed: 34192457
doi: 10.1021/acs.analchem.1c00649
pmc: PMC8922278
doi:

Substances chimiques

Lipids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

9364-9372

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Auteurs

Lucía Martín-Saiz (L)

Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

Lorena Mosteiro (L)

Service of Anatomic Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Cruces (Barakaldo) 48903, Spain.

Jon D Solano-Iturri (JD)

Service of Anatomic Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Cruces (Barakaldo) 48903, Spain.
BioCruces Health Research Institute, Cruces (Barakaldo) 48903, Spain.

Yuri Rueda (Y)

Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

Javier Martín-Allende (J)

Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

Igone Imaz (I)

Service of Anatomic Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Cruces (Barakaldo) 48903, Spain.

Iván Olano (I)

Service of Urology, Cruces University Hospital, Cruces (Barakaldo) 48903, Spain.

Begoña Ochoa (B)

Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

Olatz Fresnedo (O)

Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

José A Fernández (JA)

Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

Gorka Larrinaga (G)

BioCruces Health Research Institute, Cruces (Barakaldo) 48903, Spain.
Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.
Department of Nursing I, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena, s/n, Leioa 48940, Spain.

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Classifications MeSH