Human papilloma virus circulating tumor DNA assay predicts treatment response in recurrent/metastatic head and neck squamous cell carcinoma.

HPV circulating tumor DNA ctDNA head and neck cancer oropharyngeal cancer

Journal

Oncotarget
ISSN: 1949-2553
Titre abrégé: Oncotarget
Pays: United States
ID NLM: 101532965

Informations de publication

Date de publication:
22 Jun 2021
Historique:
received: 24 03 2021
accepted: 02 06 2021
entrez: 1 7 2021
pubmed: 2 7 2021
medline: 2 7 2021
Statut: epublish

Résumé

Despite the rising incidence of human papillomavirus related (HPV+) oropharyngeal squamous cell carcinoma (OPSCC), treatment of metastatic disease remains palliative. Even with new treatments such as immunotherapy, response rates are low and can be delayed, while even mild tumor progression in the face of an ineffective therapy can lead to rapid death. Real-time biomarkers of response to therapy could improve outcomes by guiding early change of therapy in the metastatic setting. Herein, we developed and analytically validated a new droplet digital PCR (ddPCR)-based assay for HPV16 circulating tumor DNA (ctDNA) and evaluated plasma HPV16 ctDNA for predicting treatment response in metastatic HPV+ OPSCC. We found that longitudinal changes HPV16 ctDNA correlate with treatment response and that ctDNA responses are observed earlier than conventional imaging (average 70 days, range: 35-166). With additional validation in multi-site studies, this assay may enable early identification of treatment failure, allowing patients to be directed promptly toward clinical trials or alternative therapies.

Identifiants

DOI: 10.18632/oncotarget.27992 PMID: 34194620 PMC: PMC8238244
pubmed: 34194620
doi: 10.18632/oncotarget.27992
pii: 27992
pmc: PMC8238244
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1214-1229

Informations de copyright

Copyright: © 2021 Haring et al.

Déclaration de conflit d'intérêts

CONFLICTS OF INTEREST The study team has filed an invention disclosure on the HPV16 ctDNA assay and the University of Michigan intends to file a patent on the assay technology.

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Auteurs

Catherine T Haring (CT)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.
Co-First Authors.

Chandan Bhambhani (C)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.
Co-First Authors.

Collin Brummel (C)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.

Brittany Jewell (B)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.

Emily Bellile (E)

University of Michigan, Department of Biostatistics, Ann Arbor, MI 48109, USA.

Molly E Heft Neal (ME)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.

Erin Sandford (E)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.

Ryan M Spengler (RM)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.

Apurva Bhangale (A)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.

Matthew E Spector (ME)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.
University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.

Jonathan McHugh (J)

University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.
University of Michigan, Department of Pathology, Ann Arbor, MI 48109, USA.

Mark E Prince (ME)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.

Michelle Mierzwa (M)

University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.
University of Michigan, Department of Radiation Oncology, Ann Arbor, MI 48109, USA.

Francis P Worden (FP)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.
University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.

Muneesh Tewari (M)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.
University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.
University of Michigan, Department of Biomedical Engineering, Ann Arbor, MI 48109, USA.
University of Michigan, Center for Computational Medicine and Bioinformatics, Ann Arbor, MI 48109, USA.
Co-Senior Authors.

Paul L Swiecicki (PL)

University of Michigan, Department of Internal Medicine, Division of Hematology and Oncology, Ann Arbor, MI 48109, USA.
University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.
Co-Senior Authors.

J Chad Brenner (JC)

University of Michigan, Department of Otolaryngology-Head and Neck Surgery, Ann Arbor, MI 48109, USA.
University of Michigan, Rogel Cancer Center, Ann Arbor, MI 48109, USA.
University of Michigan, Department of Pharmacology, Ann Arbor, MI 48109, USA.
Co-Senior Authors.

Classifications MeSH