miniSPIM-A Miniaturized Light-Sheet Microscope.

bacteria detection fluorescence fluctuation spectroscopy image correlation spectroscopy light-sheet microscopy optical sectioning particle detection selective plane illumination microscopy three-dimensional imaging

Journal

ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031

Informations de publication

Date de publication:
23 07 2021
Historique:
pubmed: 2 7 2021
medline: 3 8 2021
entrez: 1 7 2021
Statut: ppublish

Résumé

The miniSPIM is a miniaturized light-sheet microscope that enables imaging with optical sectioning on mobile camera devices such as smartphones and single-board computers. Applications of the miniSPIM include biosensing, field research, and education where maximum portability and robustness, low power consumption, and low cost are key. Here, it is shown how all of the components of a simple light-sheet microscope can be integrated within a footprint smaller than the average smartphone. Example applications include the quantification of the motion of microparticles and bacteria in fluids, the characterization of solvent polarity based on spectral shifts of the lipid probe Nile Red, and three-dimensional (3D) and time-lapse autofluorescence imaging of a live zebrafish embryo.

Identifiants

pubmed: 34197085
doi: 10.1021/acssensors.1c00607
pmc: PMC8314274
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2654-2663

Subventions

Organisme : NIGMS NIH HHS
ID : P41 GM103540
Pays : United States
Organisme : NIGMS NIH HHS
ID : R21 GM135493
Pays : United States

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Auteurs

Per Niklas Hedde (PN)

Beckman Laser Institute, University of California Irvine, Irvine, California 92612, United States.
Department of Pharmaceutical Sciences, University of California Irvine, Irvine, California 92697, United States.
Laboratory for Fluorescence Dynamics, University of California Irvine, Irvine, California 92697, United States.

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Classifications MeSH