Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins.
crystal structure
hyperthermophile
intein
protein engineering
protein splicing
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
05 Jun 2021
05 Jun 2021
Historique:
received:
06
05
2021
revised:
26
05
2021
accepted:
02
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
3
7
2021
Statut:
epublish
Résumé
Inteins are prevalent among extremophiles. Mini-inteins with robust splicing properties are of particular interest for biotechnological applications due to their small size. However, biochemical and structural characterization has still been limited to a small number of inteins, and only a few serve as widely used tools in protein engineering. We determined the crystal structure of a naturally occurring Pol-II mini-intein from
Identifiants
pubmed: 34198729
pii: microorganisms9061226
doi: 10.3390/microorganisms9061226
pmc: PMC8229266
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Academy of Finland
ID : 137995
Organisme : Academy of Finland
ID : 277335
Organisme : Novo Nordisk Fonden
ID : NNF17OC0025402
Organisme : Novo Nordisk Fonden
ID : NNF17OC0027550
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