Characterization of Immune Responses to SARS-CoV-2 and Other Human Pathogenic Coronaviruses Using a Multiplex Bead-Based Immunoassay.

bead-based immunoassay cross-reaction human coronaviruses multiplex immunoassay

Journal

Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355

Informations de publication

Date de publication:
07 Jun 2021
Historique:
received: 07 05 2021
revised: 31 05 2021
accepted: 02 06 2021
entrez: 2 7 2021
pubmed: 3 7 2021
medline: 3 7 2021
Statut: epublish

Résumé

Serological assays that simultaneously detect antibodies to multiple targets of SARS-CoV-2 and to other structurally related coronaviruses provide a holistic picture of antibody response patterns. Well-validated multiplex immunoassays are scarce. Here, we evaluated the performance of an 11-plex serological assay capable of detecting antibodies directed to four antigenic targets of SARS-CoV-2 and to S1 proteins of other human pathogenic coronaviruses. We used 620 well-characterized sera (

Identifiants

pubmed: 34200301
pii: vaccines9060611
doi: 10.3390/vaccines9060611
pmc: PMC8229387
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

Lancet Microbe. 2020 Aug;1(4):e151
pubmed: 33521716
Nature. 2020 Oct;586(7830):589-593
pubmed: 32785213
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
Nat Rev Immunol. 2021 Jun;21(6):340-341
pubmed: 33927376
Clin Vaccine Immunol. 2010 Dec;17(12):1875-80
pubmed: 20943876
Signal Transduct Target Ther. 2020 Oct 13;5(1):237
pubmed: 33051445
PLoS One. 2017 May 24;12(5):e0178175
pubmed: 28542611
N Engl J Med. 2020 Dec 17;383(25):2439-2450
pubmed: 33053279
Nature. 2020 Aug;584(7821):443-449
pubmed: 32668443
Vaccines (Basel). 2020 Dec 28;9(1):
pubmed: 33379160
Acta Paediatr. 2007 Mar;96(3):338-41
pubmed: 17407452
Nat Rev Immunol. 2020 Nov;20(11):709-713
pubmed: 33024281
J Med Virol. 1982;9(3):209-16
pubmed: 7097257
Int J Infect Dis. 2021 Jan;102:577-583
pubmed: 33176202
Nat Commun. 2020 Sep 17;11(1):4704
pubmed: 32943637
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651

Auteurs

Wegene Borena (W)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Janine Kimpel (J)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Melanie Gierer (M)

Thermo Fisher Scientific, Bender MedSystems GmbH, Campus Vienna Biocenter 2, 1030 Vienna, Austria.

Annika Rössler (A)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Lydia Riepler (L)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Susanne Oehler (S)

Thermo Fisher Scientific, Bender MedSystems GmbH, Campus Vienna Biocenter 2, 1030 Vienna, Austria.

Dorothee von Laer (D)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Markus Miholits (M)

Thermo Fisher Scientific, Bender MedSystems GmbH, Campus Vienna Biocenter 2, 1030 Vienna, Austria.

Classifications MeSH