Timing of Tributyrin Supplementation Differentially Modulates Gastrointestinal Inflammation and Gut Microbial Recolonization Following Murine Ileocecal Resection.
Animals
Bacteria
/ classification
Colectomy
Crohn Disease
Cytokines
/ metabolism
Dietary Supplements
Dysbiosis
Fatty Acids, Volatile
Feces
Gastrointestinal Microbiome
/ drug effects
Gastrointestinal Tract
/ immunology
Ileum
Inflammation
Inflammatory Bowel Diseases
Intestine, Large
Intestine, Small
Male
Mice
Triglycerides
/ administration & dosage
Crohn’s disease
ileocecal resection
inflammatory bowel disease
microbiome
tributyrin
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
17 Jun 2021
17 Jun 2021
Historique:
received:
01
06
2021
revised:
12
06
2021
accepted:
14
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
13
8
2021
Statut:
epublish
Résumé
Gastrointestinal surgery imparts dramatic and lasting imbalances, or dysbiosis, to the composition of finely tuned microbial ecosystems. The aim of the present study was to use a mouse ileocecal resection (ICR) model to determine if tributyrin (TBT) supplementation could prevent the onset of microbial dysbiosis or alternatively enhance the recovery of the gut microbiota and reduce gastrointestinal inflammation. Male wild-type (129 s1/SvlmJ) mice aged 8-15 weeks were separated into single cages and randomized 1:1:1:1 to each of the four experimental groups: control (CTR), preoperative TBT supplementation (PRE), postoperative TBT supplementation (POS), and combined pre- and postoperative supplementation (TOT). ICR was performed one week from baseline assessment with mice assessed at 1, 2, 3, and 4 weeks postoperatively. Primary outcomes included evaluating changes to gut microbial communities occurring from ICR to 4 weeks. A total of 34 mice that underwent ICR (CTR Taken together, the results of our work demonstrate that the timing of tributyrin supplementation differentially modulates gastrointestinal inflammation and gut microbial recolonization following murine ICR.
Sections du résumé
BACKGROUND
BACKGROUND
Gastrointestinal surgery imparts dramatic and lasting imbalances, or dysbiosis, to the composition of finely tuned microbial ecosystems. The aim of the present study was to use a mouse ileocecal resection (ICR) model to determine if tributyrin (TBT) supplementation could prevent the onset of microbial dysbiosis or alternatively enhance the recovery of the gut microbiota and reduce gastrointestinal inflammation.
METHODS
METHODS
Male wild-type (129 s1/SvlmJ) mice aged 8-15 weeks were separated into single cages and randomized 1:1:1:1 to each of the four experimental groups: control (CTR), preoperative TBT supplementation (PRE), postoperative TBT supplementation (POS), and combined pre- and postoperative supplementation (TOT). ICR was performed one week from baseline assessment with mice assessed at 1, 2, 3, and 4 weeks postoperatively. Primary outcomes included evaluating changes to gut microbial communities occurring from ICR to 4 weeks.
RESULTS
RESULTS
A total of 34 mice that underwent ICR (CTR
CONCLUSIONS
CONCLUSIONS
Taken together, the results of our work demonstrate that the timing of tributyrin supplementation differentially modulates gastrointestinal inflammation and gut microbial recolonization following murine ICR.
Identifiants
pubmed: 34204288
pii: nu13062069
doi: 10.3390/nu13062069
pmc: PMC8233937
pii:
doi:
Substances chimiques
Cytokines
0
Fatty Acids, Volatile
0
Triglycerides
0
tributyrin
S05LZ624MF
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
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