MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage.
MALDI-TOF MS
antifungal susceptibility testing
diagnosis
fungi
medical mycology
microorganism identification
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
12 Jun 2021
12 Jun 2021
Historique:
received:
04
05
2021
revised:
31
05
2021
accepted:
03
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
3
7
2021
Statut:
epublish
Résumé
The implementation of MALDI-TOF MS in medical microbiology laboratories has revolutionized practices and significantly reduced turnaround times of identification processes. However, although bacteriology quickly benefited from the contributions of this technique, adjustments were necessary to accommodate the specific characteristics of fungi. MALDI-TOF MS is now an indispensable tool in clinical mycology laboratories, both for the identification of yeasts and filamentous fungi, and other innovative uses are gradually emerging. Based on the practical experience of our medical mycology laboratory, this review will present the current uses of MALDI-TOF MS and the adaptations we implemented, to allow their practical execution in a daily routine. We will also introduce some less mainstream applications, like those for fungemia, or even still under development, as is the case for the determination of sensitivity to antifungal agents or typing methods.
Identifiants
pubmed: 34204665
pii: microorganisms9061283
doi: 10.3390/microorganisms9061283
pmc: PMC8231132
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
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