Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages.
LC-MS/MS
PMJ2-R
peritoneal macrophages
phagocytosis
proteome
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
12 Jun 2021
12 Jun 2021
Historique:
received:
19
05
2021
revised:
30
05
2021
accepted:
09
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
15
7
2021
Statut:
epublish
Résumé
In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.
Identifiants
pubmed: 34204832
pii: ijms22126323
doi: 10.3390/ijms22126323
pmc: PMC8231560
pii:
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the framework of the Russian Federation fundamental research program for the long-term period for 2021-2030» and also supported by the Ministry of Science and Higher Education of the Russian Federation
ID : agreement #14.616.21.0094, unique identifier RFMEFI61618X0094
Organisme : the Ministry of Education, Youth and Sports of the Czech Republic INTER-ACTION project
ID : LTARF 18021
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