The Influence of 5'
5′,8-cyclo-2′-deoxyadenosine (cdA)
5′,8-cyclo-2′-deoxyguanosine (cdG)
BsmAI
SspI
base excision repair (BER)
clustered lesions
restriction endonucleases
tandem lesions
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
19 Jun 2021
19 Jun 2021
Historique:
received:
29
05
2021
revised:
11
06
2021
accepted:
15
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
16
7
2021
Statut:
epublish
Résumé
Restriction endonucleases (REs) are intra-bacterial scissors that are considered tools in the fight against foreign genetic material. SspI and BsmAI, examined in this study, cleave dsDNA at their site of recognition or within a short distance of it. Both enzymes are representatives of type II REs, which have played an extremely important role in research on the genetics of organisms and molecular biology. Therefore, the study of agents affecting their activity has become highly important. Ionizing radiation may damage basic cellular mechanisms by inducing lesions in the genome, with 5',8-cyclo-2'-deoxypurines (cdPus) as a model example. Since cdPus may become components of clustered DNA lesions (CDLs), which are unfavorable for DNA repair pathways, their impact on other cellular mechanisms is worthy of attention. This study investigated the influence of cdPus on the elements of the bacterial restriction-modification system. In this study, it was shown that cdPus present in DNA affect the activity of REs. SspI was blocked by any cdPu lesion present at the enzyme's recognition site. When lesions were placed near the recognition sequence, the SspI was inhibited up to 46%. Moreover, (5'
Identifiants
pubmed: 34205449
pii: molecules26123750
doi: 10.3390/molecules26123750
pmc: PMC8234751
pii:
doi:
Substances chimiques
Deoxyadenosines
0
Oligonucleotides
0
DNA
9007-49-2
DNA Restriction Enzymes
EC 3.1.21.-
endodeoxyribonuclease BsmAI
EC 3.1.21.-
endodeoxyribonuclease SspI
EC 3.1.21.-
Deoxyribonucleases, Type II Site-Specific
EC 3.1.21.4
Deoxyguanosine
G9481N71RO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Narodowe Centrum Nauki
ID : 2016/23/B/NZ7/03367
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