How to Obtain a High Quality ctDNA in Lymphoma Patients: Preanalytical Tips and Tricks.
ctDNA
lymphoma
preanalytical
Journal
Pharmaceuticals (Basel, Switzerland)
ISSN: 1424-8247
Titre abrégé: Pharmaceuticals (Basel)
Pays: Switzerland
ID NLM: 101238453
Informations de publication
Date de publication:
26 Jun 2021
26 Jun 2021
Historique:
received:
11
05
2021
revised:
22
06
2021
accepted:
24
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
3
7
2021
Statut:
epublish
Résumé
The analysis of circulating tumor DNA (ctDNA) released by tumor cells holds great promise for patients with lymphoma, to refine the diagnostic procedure, clarify the prognosis, monitor the response to treatment, and detect relapses earlier. One of the main challenges of the coming years is to adapt techniques from highly specialized translational teams to routine laboratories as this requires a careful technical and clinical validation, and we have to achieve this as fast as possible to transform a promising biomarker into a routine analysis to have a direct consequence on patient care. Whatever the analytical technology used, the prerequisite is to obtain high yields of ctDNA of optimal quality. In this review, we propose a step-by-step description of the preanalytical process to obtain high-quality ctDNA, emphasizing the technical choices that need to be made and the experimental data that can support these choices.
Identifiants
pubmed: 34206947
pii: ph14070617
doi: 10.3390/ph14070617
pmc: PMC8308879
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Références
Biochim Biophys Acta. 2007 Jan;1775(1):181-232
pubmed: 17137717
Prenat Diagn. 2007 Sep;27(9):824-9
pubmed: 17604339
Hematology Am Soc Hematol Educ Program. 2019 Dec 6;2019(1):47-52
pubmed: 31808836
Sci Transl Med. 2016 Nov 9;8(364):364ra155
pubmed: 27831904
Cancer Biomark. 2018;23(3):427-436
pubmed: 30223392
Clin Chim Acta. 2001 Nov;313(1-2):139-42
pubmed: 11694251
EBioMedicine. 2019 Nov;49:284-290
pubmed: 31648993
Pathol Oncol Res. 2017 Jul;23(3):699-706
pubmed: 28035516
Cancer Genet. 2018 Dec;228-229:143-150
pubmed: 29567030
Clin Biochem. 2015 Oct;48(15):957-61
pubmed: 25889059
Clin Chem. 2016 Dec;62(12):1672-1673
pubmed: 27737896
Anal Biochem. 2018 Feb 1;542:34-39
pubmed: 29137972
Ann N Y Acad Sci. 2008 Aug;1137:98-107
pubmed: 18837931
J Mol Diagn. 2004 May;6(2):101-7
pubmed: 15096565
Nat Commun. 2015 Nov 10;6:8839
pubmed: 26554728
Blood Adv. 2021 Jun 8;5(11):2505-2514
pubmed: 34047776
Sci Rep. 2016 Dec 09;6:38639
pubmed: 27934896
Adv Clin Chem. 2019;89:131-188
pubmed: 30797468
Clin Chem. 2005 Jan;51(1):113-8
pubmed: 15539466
Haematologica. 2021 Feb 01;106(2):513-521
pubmed: 32079701
Breast Cancer Res Treat. 2019 Sep;177(2):447-455
pubmed: 31236809
Clin Cancer Res. 2021 Jan 15;27(2):513-521
pubmed: 33122345
Clin Chem. 2015 Mar;61(3):514-22
pubmed: 25605683
Ann N Y Acad Sci. 2008 Aug;1137:135-9
pubmed: 18837937
Clin Biochem. 2013 Oct;46(15):1561-5
pubmed: 23769817
J Natl Cancer Inst. 2005 Dec 21;97(24):1848-50
pubmed: 16368947
Nat Med. 2020 Jul;26(7):1114-1124
pubmed: 32483360
Nat Biotechnol. 2021 Apr 12;:
pubmed: 33846644
Crit Rev Clin Lab Sci. 2019 Dec 22;:1-17
pubmed: 31865831
Biomol Detect Quantif. 2019 Mar 18;17:100087
pubmed: 30923679
Cancer Res. 2001 Feb 15;61(4):1659-65
pubmed: 11245480
Clin Chem. 2000 Aug;46(8 Pt 1):1078-84
pubmed: 10926886
Crit Rev Clin Lab Sci. 2020 Nov;57(7):484-507
pubmed: 32393081
J Gen Intern Med. 2005 Jun;20(6):520-4
pubmed: 15987327
Sci Transl Med. 2014 Feb 19;6(224):224ra24
pubmed: 24553385
J Mol Diagn. 2017 Jan;19(1):162-168
pubmed: 27865784
C R Seances Soc Biol Fil. 1948 Feb;142(3-4):241-3
pubmed: 18875018
Clin Cancer Res. 2020 Jul 1;26(13):3104-3109
pubmed: 32122922
Mol Oncol. 2019 Oct;13(10):2098-2106
pubmed: 31306545
Clin Chem. 2021 Jan 30;67(2):434-435
pubmed: 33280007
Cell. 2019 Jul 25;178(3):699-713.e19
pubmed: 31280963
Blood. 2014 Apr 3;123(14):2139-47
pubmed: 24501221
Clin Chim Acta. 2013 Sep 23;424:222-30
pubmed: 23727028
J Thorac Oncol. 2016 Oct;11(10):1690-700
pubmed: 27468937
Clin Chem Lab Med. 2018 Jan 26;56(2):220-228
pubmed: 28841569
Nat Med. 2019 Dec;25(12):1928-1937
pubmed: 31768066
J Clin Oncol. 2018 Oct 1;36(28):2845-2853
pubmed: 30125215