P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation.
ATP sensitization
P2X7
anoctamin
cell permeabilization
ion channel
purinergic receptor
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Jun 2021
18 Jun 2021
Historique:
received:
18
05
2021
revised:
04
06
2021
accepted:
07
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
20
7
2021
Statut:
epublish
Résumé
P2X7 receptors (P2X7) are cationic channels involved in many diseases. Following their activation by extracellular ATP, distinct signaling pathways are triggered, which lead to various physiological responses such as the secretion of pro-inflammatory cytokines or the modulation of cell death. P2X7 also exhibit unique behaviors, such as "macropore" formation, which corresponds to enhanced large molecule cell membrane permeability and current facilitation, which is caused by prolonged activation. These two phenomena have often been confounded but, thus far, no clear mechanisms have been resolved. Here, by combining different approaches including whole-cell and single-channel recordings, pharmacological and biochemical assays, CRISPR/Cas9 technology and cell imaging, we provide evidence that current facilitation and macropore formation involve functional complexes comprised of P2X7 and TMEM16, a family of Ca
Identifiants
pubmed: 34207150
pii: ijms22126542
doi: 10.3390/ijms22126542
pmc: PMC8234106
pii:
doi:
Substances chimiques
Anoctamins
0
Receptors, Purinergic P2X7
0
Adenosine Triphosphate
8L70Q75FXE
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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