Structural Analysis of a Genetically Encoded FRET Biosensor by SAXS and MD Simulations.
coarse-grained molecular dynamics (MD)
glucose sensor
green fluorescence protein (GFP)
single-molecule FRET
small angle X-ray scattering (SAXS)
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
16 Jun 2021
16 Jun 2021
Historique:
received:
14
05
2021
revised:
11
06
2021
accepted:
11
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
7
7
2021
Statut:
epublish
Résumé
Inspired by the modular architecture of natural signaling proteins, ligand binding proteins are equipped with two fluorescent proteins (FPs) in order to obtain Förster resonance energy transfer (FRET)-based biosensors. Here, we investigated a glucose sensor where the donor and acceptor FPs were attached to a glucose binding protein using a variety of different linker sequences. For three resulting sensor constructs the corresponding glucose induced conformational changes were measured by small angle X-ray scattering (SAXS) and compared to recently published single molecule FRET results (Höfig et al.,
Identifiants
pubmed: 34208740
pii: s21124144
doi: 10.3390/s21124144
pmc: PMC8234384
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 05K16PA1
Références
Proteins. 1999 Feb 15;34(3):369-82
pubmed: 10024023
J Phys Chem B. 2012 Jun 14;116(23):6880-8
pubmed: 22497217
J Appl Crystallogr. 2017 Jun 26;50(Pt 4):1212-1225
pubmed: 28808438
Bioinformatics. 2013 Nov 1;29(21):2795-6
pubmed: 24021379
Curr Opin Struct Biol. 2004 Aug;14(4):495-504
pubmed: 15313245
Biophys J. 1977 Dec;20(3):289-305
pubmed: 922121
ACS Sens. 2019 Dec 27;4(12):3089-3091
pubmed: 31842538
Methods Enzymol. 2017;589:1-49
pubmed: 28336060
Anal Chem. 2009 Aug 1;81(15):5972-9
pubmed: 19552419
Protein Sci. 2002 Nov;11(11):2655-75
pubmed: 12381848
Protein Sci. 2007 Jun;16(6):1032-41
pubmed: 17473016
Biophys J. 2014 Oct 21;107(8):1913-1923
pubmed: 25418172
Biophys J. 2017 Oct 17;113(8):1731-1737
pubmed: 29045867
J Comput Chem. 2005 Dec;26(16):1701-18
pubmed: 16211538
Chembiochem. 2007 Jul 9;8(10):1119-21
pubmed: 17525917
Structure. 2007 May;15(5):515-23
pubmed: 17502097
Microb Cell Fact. 2019 Aug 21;18(1):143
pubmed: 31434564
Biophys J. 2012 May 16;102(10):2401-10
pubmed: 22677394
Biochemistry. 1994 Jun 7;33(22):6739-49
pubmed: 8204609
Sci Adv. 2019 Aug 07;5(8):eaaw4988
pubmed: 31457088
Sensors (Basel). 2014 Jul 02;14(7):11691-713
pubmed: 24991940
Proteins. 1995 Mar;21(3):167-95
pubmed: 7784423
Annu Rev Phys Chem. 1997;48:545-600
pubmed: 9348663
Protein Sci. 2005 Sep;14(9):2304-14
pubmed: 16131659
J Vis Exp. 2017 Jan 5;(119):
pubmed: 28117806
Biophys J. 2012 Jun 20;102(12):2866-75
pubmed: 22735537
J Biol Chem. 2002 Dec 27;277(52):50573-8
pubmed: 12370172
J Chem Phys. 2018 Mar 28;148(12):123321
pubmed: 29604831
ACS Chem Biol. 2014 Dec 19;9(12):2708-17
pubmed: 25317665
Nat Commun. 2012 Mar 20;3:751
pubmed: 22434194
Sensors (Basel). 2015 Oct 16;15(10):26281-314
pubmed: 26501285
Sci Rep. 2018 May 8;8(1):7204
pubmed: 29739979
ACS Sens. 2018 Aug 24;3(8):1462-1470
pubmed: 29979038
J Appl Crystallogr. 2012 Mar 15;45(Pt 2):342-350
pubmed: 25484842