Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes.

Blotting, Southern Nucleic Acid Hybridization Nucleic Acids

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 07 2021
Historique:
entrez: 2 7 2021
pubmed: 3 7 2021
medline: 14 1 2022
Statut: epublish

Résumé

In this protocol, restriction fragments that have been transferred to a membrane by Southern blotting are hybridized to a labeled probe. Methods for stripping the probe from the membrane are also included.

Identifiants

DOI: 10.1101/pdb.prot100495 PMID: 34210770
pubmed: 34210770
pii: 2021/7/pdb.prot100495
doi: 10.1101/pdb.prot100495
doi:

Substances chimiques

Nucleic Acids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2021 Cold Spring Harbor Laboratory Press.

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Technique de Southern › Southern Hybridization of Radiolabeled Probes...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Hybridation d'acides nucléiques › Southern Hybridization of Radiolabeled Probes...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › Southern Hybridization of Radiolabeled Probes...