Covalent Chemistry-Mediated Multimarker Purification of Circulating Tumor Cells Enables Noninvasive Detection of Molecular Signatures of Hepatocellular Carcinoma.

circulating tumor cells click chemistry hepatocellular carcinoma nanosubstrate transcriptome profiling

Journal

Advanced materials technologies
ISSN: 2365-709X
Titre abrégé: Adv Mater Technol
Pays: United States
ID NLM: 101717732

Informations de publication

Date de publication:
May 2021
Historique:
entrez: 2 7 2021
pubmed: 3 7 2021
medline: 3 7 2021
Statut: ppublish

Résumé

Transcriptomic profiling of tumor tissues introduces a large database, which has led to improvements in the ability of cancer diagnosis, treatment, and prevention. However, performing tumor transcriptomic profiling in the clinical setting is very challenging since the procurement of tumor tissues is inherently limited by invasive sampling procedures. Here, we demonstrated the feasibility of purifying hepatocellular carcinoma (HCC) circulating tumor cells (CTCs) from clinical patient samples with improved molecular integrity using Click Chips in conjunction with a multimarker antibody cocktail. The purified CTCs were then subjected to mRNA profiling by NanoString nCounter platform, targeting 64 HCC-specific genes, which were generated from an integrated data analysis framework with 8 tissue-based prognostic gene signatures from 7 publicly available HCC transcriptomic studies. After bioinformatics analysis and comparison, the HCC CTC-derived gene signatures showed high concordance with HCC tissue-derived gene signatures from TCGA database, suggesting that HCC CTCs purified by Click Chips could enable the translation of HCC tissue molecular profiling into a noninvasive setting.

Identifiants

DOI: 10.1002/admt.202001056 PMID: 34212072 PMC: PMC8240468
pubmed: 34212072
doi: 10.1002/admt.202001056
pmc: PMC8240468
mid: NIHMS1707763
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : NCI NIH HHS
ID : R21 CA216807
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA218356
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA235340
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA255727
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB026421
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA240887
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA198900
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA253651
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA246304
Pays : United States

Déclaration de conflit d'intérêts

Conflict of Interest The authors declare no conflict of interest.

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Auteurs

Na Sun (N)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Yi-Te Lee (YT)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Minhyung Kim (M)

Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Jasmine J Wang (JJ)

Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Ceng Zhang (C)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Pai-Chi Teng (PC)

Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Dongping Qi (D)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Ryan Y Zhang (RY)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Benjamin V Tran (BV)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Yue Tung Lee (YT)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Jinglei Ye (J)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Juvelyn Palomique (J)

Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Nicholas N Nissen (NN)

Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Steven-Huy B Han (SB)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Saeed Sadeghi (S)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Richard S Finn (RS)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Sammy Saab (S)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Ronald W Busuttil (RW)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Edwin M Posadas (EM)

Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Li Liang (L)

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong Province, P.R. China.

Renjun Pei (R)

Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Suzhou 215123, P.R. China.

Ju Dong Yang (JD)

Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Sungyong You (S)

Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Vatche G Agopian (VG)

Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.

Hsian-Rong Tseng (HR)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Yazhen Zhu (Y)

California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.

Classifications MeSH