Exosomes mediate horizontal transmission of viral pathogens from insect vectors to plant phloem.
exosome
infectious disease
insect vectors
microbiology
multivesicular bodies
plant phloem
salivary glands
viral transmission
virus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
02 07 2021
02 07 2021
Historique:
received:
04
11
2020
accepted:
21
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
23
9
2021
Statut:
epublish
Résumé
Numerous piercing-sucking insects can horizontally transmit viral pathogens together with saliva to plant phloem, but the mechanism remains elusive. Here, we report that an important rice reovirus has hijacked small vesicles, referred to as exosomes, to traverse the apical plasmalemma into saliva-stored cavities in the salivary glands of leafhopper vectors. Thus, virions were horizontally transmitted with exosomes into rice phloem to establish the initial plant infection during vector feeding. The purified exosomes secreted from cultured leafhopper cells were enriched with virions. Silencing the exosomal secretion-related small GTPase Rab27a or treatment with the exosomal biogenesis inhibitor GW4869 strongly prevented viral exosomal release in vivo and in vitro. Furthermore, the specific interaction of the 15-nm-long domain of the viral outer capsid protein with Rab5 induced the packaging of virions in exosomes, ultimately activating the Rab27a-dependent exosomal release pathway. We thus anticipate that exosome-mediated viral horizontal transmission is the conserved strategy hijacked by vector-borne viruses.
Identifiants
pubmed: 34214032
doi: 10.7554/eLife.64603
pii: 64603
pmc: PMC8253596
doi:
pii:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31772124
Organisme : National Natural Science Foundation of China
ID : 31920103014
Organisme : National Natural Science Foundation of China
ID : 31972239
Informations de copyright
© 2021, Chen et al.
Déclaration de conflit d'intérêts
QC, YL, JR, PZ, MC, DJ, HC, TW No competing interests declared
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