SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor.
Aptamer
Plastic optical fiber (POF)
Polyethyleneglycol (PEG)
Sars-CoV-2
Self assembled monolayer (SAM)
Surface plasmon resonance (SPR)
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Oct 2021
01 Oct 2021
Historique:
received:
07
03
2021
revised:
11
05
2021
accepted:
13
05
2021
entrez:
3
7
2021
pubmed:
4
7
2021
medline:
8
7
2021
Statut:
ppublish
Résumé
A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 ÷ 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.
Identifiants
pubmed: 34215035
pii: S0039-9140(21)00453-7
doi: 10.1016/j.talanta.2021.122532
pmc: PMC8133803
pii:
doi:
Substances chimiques
Plastics
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122532Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Références
Heliyon. 2020 Sep;6(9):e04943
pubmed: 32954038
Anal Bioanal Chem. 2015 May;407(14):3883-97
pubmed: 25616701
J Clin Med. 2020 May 18;9(5):
pubmed: 32443459
Pharmaceuticals (Basel). 2020 Jul 22;13(8):
pubmed: 32707768
Heliyon. 2020 Sep;6(9):e05007
pubmed: 32984620
Viruses. 2015 Feb 16;7(2):751-80
pubmed: 25690797
J Biosci Bioeng. 2011 Dec;112(6):535-40
pubmed: 21920814
Chem Commun (Camb). 2020 Sep 11;56(70):10235-10238
pubmed: 32756614
ACS Omega. 2021 Feb 23;6(9):6404-6413
pubmed: 33718731
Sensors (Basel). 2011;11(12):11752-60
pubmed: 22247691
Trends Analyt Chem. 2020 Dec;133:116067
pubmed: 33052154
Sci Rep. 2019 Dec 10;9(1):18740
pubmed: 31822733
Diagnostics (Basel). 2020 Jun 26;10(6):
pubmed: 32604919
Pathogens. 2020 Jun 28;9(7):
pubmed: 32605194
Viruses. 2020 May 26;12(6):
pubmed: 32466458
ACS Nano. 2020 Jun 23;14(6):7617-7627
pubmed: 32437124
ACS Nano. 2020 May 26;14(5):5268-5277
pubmed: 32281785
Biosensors (Basel). 2017 Jun 21;7(2):
pubmed: 28635665
Langmuir. 2011 Jan 18;27(2):678-85
pubmed: 21142210
Biosens Bioelectron. 2019 Jun 15;135:102-110
pubmed: 31004920
Anal Chem. 2017 Jun 20;89(12):6781-6786
pubmed: 28558471
ACS Sens. 2020 Jun 26;5(6):1506-1513
pubmed: 32482077
ACS Nano. 2020 Apr 28;14(4):5135-5142
pubmed: 32293168
Biosens Bioelectron. 2020 Mar 15;152:112010
pubmed: 31941618
Pharmaceuticals (Basel). 2016 Dec 16;9(4):
pubmed: 27999271
Anal Chem. 2020 Jul 21;92(14):9895-9900
pubmed: 32551560
Talanta. 2021 Jan 1;221:121452
pubmed: 33076075
Talanta. 2020 Apr 1;210:120666
pubmed: 31987191
Sensors (Basel). 2021 Mar 01;21(5):
pubmed: 33804378
Chem Sci. 2020 Oct 12;11(44):12157-12164
pubmed: 34123223
Biosens Bioelectron. 2013 May 15;43:245-51
pubmed: 23318547
ACS Sens. 2020 Sep 25;5(9):2663-2678
pubmed: 32786383
Front Microbiol. 2019 Jul 03;10:1462
pubmed: 31333603
Sci Rep. 2017 Feb 21;7:42794
pubmed: 28220811
Diagnostics (Basel). 2020 Jul 04;10(7):
pubmed: 32635444
Anal Chem. 2017 Dec 5;89(23):12743-12748
pubmed: 29120623
Virol Sin. 2020 Jun;35(3):351-354
pubmed: 32451881
Nat Nanotechnol. 2020 Aug;15(8):618-621
pubmed: 32728083
Emerg Microbes Infect. 2020 Dec;9(1):1671-1681
pubmed: 32623963
ChemistrySelect. 2020 Jul 7;5(25):7521-7533
pubmed: 32835089
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
ACS Nano. 2020 Apr 28;14(4):3822-3835
pubmed: 32223179