Decentralized Investigation of Bacterial Outbreaks Based on Hashed cgMLST.

Salmonella WGS typing cgMLST comparative microbial genomics molecular surveillance

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2021
Historique:
received: 19 01 2021
accepted: 25 03 2021
entrez: 5 7 2021
pubmed: 6 7 2021
medline: 6 7 2021
Statut: epublish

Résumé

Whole-genome sequencing (WGS)-based outbreak investigation has proven to be a valuable method for the surveillance of bacterial pathogens. Its utility has been successfully demonstrated using both gene-by-gene (cgMLST or wgMLST) and single-nucleotide polymorphism (SNP)-based approaches. Among the obstacles of implementing a WGS-based routine surveillance is the need for an exchange of large volumes of sequencing data, as well as a widespread reluctance to share sequence and metadata in public repositories, together with a lacking standardization of suitable bioinformatic tools and workflows. To address these issues, we present

Identifiants

pubmed: 34220740
doi: 10.3389/fmicb.2021.649517
pmc: PMC8244591
doi:

Types de publication

Journal Article

Langues

eng

Pagination

649517

Informations de copyright

Copyright © 2021 Deneke, Uelze, Brendebach, Tausch and Malorny.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

Genome Res. 2018 Sep;28(9):1395-1404
pubmed: 30049790
Nat Rev Microbiol. 2013 Oct;11(10):728-36
pubmed: 23979428
J Clin Microbiol. 2011 Nov;49(11):3997-4000
pubmed: 21918028
J Clin Microbiol. 2019 Dec 23;58(1):
pubmed: 31666367
Genome Res. 2020 Jan;30(1):138-152
pubmed: 31809257
PLoS One. 2016 Jan 22;11(1):e0147101
pubmed: 26800248
Bioinformatics. 2015 Nov 15;31(22):3718-20
pubmed: 26209431
Food Microbiol. 2018 May;71:46-54
pubmed: 29366468
Front Microbiol. 2020 Sep 11;11:573972
pubmed: 33013811
Foodborne Pathog Dis. 2019 Jul;16(7):504-512
pubmed: 31246502
Tuberculosis (Edinb). 2019 Mar;115:81-88
pubmed: 30948181
Appl Environ Microbiol. 2020 Feb 18;86(5):
pubmed: 31862714
Nucleic Acids Res. 2021 Jan 8;49(D1):D660-D666
pubmed: 33068420
J Clin Microbiol. 2006 Jul;44(7):2524-32
pubmed: 16825375
One Health Outlook. 2020 Feb 18;2:3
pubmed: 33829127
Bioinformatics. 2012 Oct 1;28(19):2520-2
pubmed: 22908215
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
Microb Genom. 2018 Feb;4(2):
pubmed: 29319471
BMC Bioinformatics. 2012 May 08;13:87
pubmed: 22568821
Food Microbiol. 2019 Jun;79:96-115
pubmed: 30621881
PLoS One. 2008;3(11):e3696
pubmed: 19002246
Microb Genom. 2018 Mar;4(3):
pubmed: 29543149
Clin Microbiol Rev. 2016 Oct;29(4):837-57
pubmed: 27559074
Microb Genom. 2020 Mar;6(3):
pubmed: 32101514
Nat Methods. 2018 Jul;15(7):475-476
pubmed: 29967506
Front Microbiol. 2018 Jul 10;9:1482
pubmed: 30042741
Front Microbiol. 2019 Oct 24;10:2413
pubmed: 31708892
Bioinformatics. 2018 Sep 1;34(17):3028-3029
pubmed: 29659710
Bioinformatics. 2021 Apr 06;:
pubmed: 33823553
Microb Genom. 2021 Feb;7(2):
pubmed: 33245688
Front Microbiol. 2021 Feb 10;12:626941
pubmed: 33643254
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
Genes (Basel). 2021 Apr 26;12(5):
pubmed: 33926025
Microb Genom. 2021 Jan;7(1):
pubmed: 33275089

Auteurs

Carlus Deneke (C)

Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Laura Uelze (L)

Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Holger Brendebach (H)

Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Simon H Tausch (SH)

Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Burkhard Malorny (B)

Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Classifications MeSH