Children With Asthma Have Impaired Innate Immunity and Increased Numbers of Type 2 Innate Lymphoid Cells Compared With Healthy Controls.
asthma
children
innate immune response
innate lymphoid cells
lung function
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
05
02
2021
accepted:
17
05
2021
entrez:
5
7
2021
pubmed:
6
7
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Asthma is the most frequent cause of hospitalisation among children; however, little is known regarding the effects of asthma on immune responses in children. The present study aimed to evaluate cytokine responses of peripheral blood mononuclear cells (PBMCs), PBMC composition and lung function in children with and without asthma. Using a case-control design, we compared 48 children with asthma aged 3-11 years with 14 age-matched healthy controls. PBMC composition and cytokine production including interferon (IFN)-γ, interleukin (IL)-1β, IL-5 and lL-6 following stimulation with rhinovirus-1B (RV1B), house dust mite (HDM) and lipopolysaccharide (LPS) were measured. Lung function was assessed using impulse oscillometry and nitrogen multiple breath washout. The frequency of group 2 innate lymphoid cells were significantly higher in asthmatics and PBMCs from asthmatics had deficient IFN-γ production in response to both RV1B and LPS compared with controls (P<0.01). RV1B-induced IL-1β response and HDM-stimulated IL-5 production was higher in asthmatics than controls (P<0.05). In contrast, IL-1β and IL-6 were significantly reduced in response to HDM and LPS in asthmatics compared to controls (P<0.05). Children with asthma also had reduced pulmonary function, indicated by lower respiratory reactance as well as higher area of-reactance and lung clearance index values compared with controls (P<0.05). Our study indicates that children with asthma have a reduced lung function in concert with impaired immune responses and altered immune cell subsets. Improving our understanding of immune responses to viral and bacterial infection in childhood asthma can help to tailor management of the disease.
Sections du résumé
Background
Asthma is the most frequent cause of hospitalisation among children; however, little is known regarding the effects of asthma on immune responses in children.
Objective
The present study aimed to evaluate cytokine responses of peripheral blood mononuclear cells (PBMCs), PBMC composition and lung function in children with and without asthma.
Methods
Using a case-control design, we compared 48 children with asthma aged 3-11 years with 14 age-matched healthy controls. PBMC composition and cytokine production including interferon (IFN)-γ, interleukin (IL)-1β, IL-5 and lL-6 following stimulation with rhinovirus-1B (RV1B), house dust mite (HDM) and lipopolysaccharide (LPS) were measured. Lung function was assessed using impulse oscillometry and nitrogen multiple breath washout.
Results
The frequency of group 2 innate lymphoid cells were significantly higher in asthmatics and PBMCs from asthmatics had deficient IFN-γ production in response to both RV1B and LPS compared with controls (P<0.01). RV1B-induced IL-1β response and HDM-stimulated IL-5 production was higher in asthmatics than controls (P<0.05). In contrast, IL-1β and IL-6 were significantly reduced in response to HDM and LPS in asthmatics compared to controls (P<0.05). Children with asthma also had reduced pulmonary function, indicated by lower respiratory reactance as well as higher area of-reactance and lung clearance index values compared with controls (P<0.05).
Conclusion
Our study indicates that children with asthma have a reduced lung function in concert with impaired immune responses and altered immune cell subsets. Improving our understanding of immune responses to viral and bacterial infection in childhood asthma can help to tailor management of the disease.
Identifiants
pubmed: 34220812
doi: 10.3389/fimmu.2021.664668
pmc: PMC8248177
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
664668Informations de copyright
Copyright © 2021 Hosseini, Berthon, Starkey, Collison, McLoughlin, Williams, Nichol, Wark, Jensen, Da Silva Sena, Baines, Mattes and Wood.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Allergy Clin Immunol. 2005 Nov;116(5):1053-7
pubmed: 16275375
Int Arch Allergy Immunol. 2011;155 Suppl 1:27-33
pubmed: 21646792
Clin Exp Allergy. 2014 Jan;44(1):47-57
pubmed: 24355018
Clin Exp Allergy. 1999 Jun;29(6):780-5
pubmed: 10336594
Clin Exp Allergy. 2014 Apr;44(4):499-507
pubmed: 24341600
Respirology. 2009 Sep;14(7):1035-41
pubmed: 19740263
Am J Respir Cell Mol Biol. 2009 May;40(5):610-9
pubmed: 18978302
PLoS Pathog. 2020 Jul 13;16(7):e1008651
pubmed: 32658914
PLoS One. 2014 May 29;9(5):e97995
pubmed: 24875149
Am J Respir Crit Care Med. 2005 Dec 1;172(11):1371-7
pubmed: 16141442
Cell Rep. 2014 May 22;7(4):1130-42
pubmed: 24813893
J Allergy Clin Immunol. 2016 Apr;137(4):1238-1246.e13
pubmed: 26581916
Front Immunol. 2018 Aug 16;9:1895
pubmed: 30174671
Allergy. 2020 Aug;75(8):2005-2019
pubmed: 32086822
Curr Allergy Asthma Rep. 2016 Nov;16(11):78
pubmed: 27796793
Int J Mol Sci. 2020 May 26;21(11):
pubmed: 32466530
Thorax. 2002 Apr;57(4):328-32
pubmed: 11923551
Eur Respir J. 2017 Feb 2;49(2):
pubmed: 27836955
Trends Immunol. 2013 Nov;34(11):540-7
pubmed: 24035576
J Allergy Clin Immunol. 2008 Oct;122(4):671-682
pubmed: 19014757
ESMO Open. 2019 Feb 27;4(1):e000477
pubmed: 30964127
Allergy. 2004 May;59(5):469-78
pubmed: 15080825
Nat Med. 2006 Sep;12(9):1023-6
pubmed: 16906156
Ann Am Thorac Soc. 2017 Sep;14(9):1436-1442
pubmed: 28481640
Am J Respir Cell Mol Biol. 2016 Nov;55(5):675-683
pubmed: 27314535
Proc Biol Sci. 2015 Dec 22;282(1821):20143085
pubmed: 26702035
J Allergy Clin Immunol. 2012 Oct;130(4):869-76.e2
pubmed: 22770265
J Allergy Clin Immunol. 2013 Mar;131(3):718-23
pubmed: 23146376
Semin Immunol. 2013 Feb;25(1):2-11
pubmed: 23694705
Clin Diagn Lab Immunol. 2002 May;9(3):530-43
pubmed: 11986256
Sci Rep. 2014 Nov 24;4:7176
pubmed: 25417801
J Allergy Clin Immunol. 2000 Jul;106(1 Pt 1):84-91
pubmed: 10887310
Ann Am Thorac Soc. 2016 Mar;13 Suppl 1:S55-63
pubmed: 27027954
Acta Biomed. 2018 Jun 14;89(2):148-156
pubmed: 29957746
Eur J Immunol. 2016 Apr;46(4):795-806
pubmed: 26891006
Thorax. 2009 Jan;64(1):33-7
pubmed: 18678703
Eur Respir J. 1994 Jun;7(6):1096-102
pubmed: 7925879
Cytokine. 2015 Dec;76(2):334-342
pubmed: 26239413
J Allergy Clin Immunol. 2003 Aug;112(2):317-22
pubmed: 12897737
Eur Respir Rev. 2019 Nov 20;28(154):
pubmed: 31748419
Thorax. 2012 Mar;67(3):209-14
pubmed: 21917654
J Allergy Clin Immunol. 2014 Sep;134(3):671-678.e4
pubmed: 25171868
Eur Respir J. 2014 Dec;44(6):1666-81
pubmed: 25234802
J Clin Immunol. 2009 May;29(3):330-42
pubmed: 19067129
Ital J Pediatr. 2017 Jul 15;43(1):60
pubmed: 28709466
Respiration. 2016;91(2):107-14
pubmed: 26756585
J Exp Med. 2005 Mar 21;201(6):937-47
pubmed: 15781584
Respir Med. 2014 Sep;108(9):1254-9
pubmed: 25127180
PLoS Pathog. 2014 Dec 11;10(12):e1004556
pubmed: 25503988
Eur Respir J. 2010 Aug;36(2):438-45
pubmed: 20675781
J Asthma. 2013 Mar;50(2):198-203
pubmed: 23294170
J Immunol. 2010 Jun 1;184(11):5999-6006
pubmed: 20410486