Phenotypic and genotypic antimicrobial resistance in clinical anaerobic isolates from India.
Journal
JAC-antimicrobial resistance
ISSN: 2632-1823
Titre abrégé: JAC Antimicrob Resist
Pays: England
ID NLM: 101765283
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
11
12
2020
accepted:
10
03
2021
entrez:
5
7
2021
pubmed:
6
7
2021
medline:
6
7
2021
Statut:
epublish
Résumé
Antimicrobial resistance (AMR) in anaerobes remains a neglected field. The laborious procedures, non-compliance with the standard methodology and differences in interpretive breakpoints add variation in resistance data. To assess the phenotypic and genotypic resistance among clinically important anaerobes to six antibiotics frequently used as empirical therapy for anaerobic infections. A total of 150 anaerobic isolates were recovered from clinical specimens. The antimicrobial susceptibility was determined by the breakpoint agar dilution method as per CLSI guidelines. The presence of genes encoding resistance to metronidazole ( This is a first study of its kind from the Indian subcontinent looking at the AMR and associated genes in anaerobes. Resistance to metronidazole, clindamycin, imipenem, piperacillin/tazobactam and cefoxitin was 32.6%, 42.6%, 0.6%, 38% and 35.3%, respectively. No resistance was observed to chloramphenicol. The The possibility of isolates carrying AMR genes to become resistant to antibiotics by acquisition of IS elements mandates attention to periodically monitor the resistance patterns and geographic distribution of these genes and IS elements to understand the trends of AMR in anaerobes.
Sections du résumé
BACKGROUND
BACKGROUND
Antimicrobial resistance (AMR) in anaerobes remains a neglected field. The laborious procedures, non-compliance with the standard methodology and differences in interpretive breakpoints add variation in resistance data.
OBJECTIVES
OBJECTIVE
To assess the phenotypic and genotypic resistance among clinically important anaerobes to six antibiotics frequently used as empirical therapy for anaerobic infections.
METHODS
METHODS
A total of 150 anaerobic isolates were recovered from clinical specimens. The antimicrobial susceptibility was determined by the breakpoint agar dilution method as per CLSI guidelines. The presence of genes encoding resistance to metronidazole (
RESULTS
RESULTS
This is a first study of its kind from the Indian subcontinent looking at the AMR and associated genes in anaerobes. Resistance to metronidazole, clindamycin, imipenem, piperacillin/tazobactam and cefoxitin was 32.6%, 42.6%, 0.6%, 38% and 35.3%, respectively. No resistance was observed to chloramphenicol. The
CONCLUSIONS
CONCLUSIONS
The possibility of isolates carrying AMR genes to become resistant to antibiotics by acquisition of IS elements mandates attention to periodically monitor the resistance patterns and geographic distribution of these genes and IS elements to understand the trends of AMR in anaerobes.
Identifiants
pubmed: 34223113
doi: 10.1093/jacamr/dlab044
pii: dlab044
pmc: PMC8210138
doi:
Types de publication
Journal Article
Langues
eng
Pagination
dlab044Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
Références
Anaerobe. 2015 Jun;33:132-6
pubmed: 25800668
GMS Hyg Infect Control. 2017 Aug 17;12:Doc13
pubmed: 28840093
Eur J Clin Microbiol Infect Dis. 1992 Apr;11(4):292-304
pubmed: 1396748
Clinics (Sao Paulo). 2011;66(4):543-7
pubmed: 21655744
J Antimicrob Chemother. 2004 Jul;54(1):109-16
pubmed: 15190033
J Clin Microbiol. 2000 Jun;38(6):2429-33
pubmed: 10835024
Ann Lab Med. 2015 Sep;35(5):479-86
pubmed: 26206683
Anaerobe. 2020 Jun;63:102200
pubmed: 32247001
Clin Microbiol Infect. 2005 Oct;11(10):820-4
pubmed: 16153256
Clin Microbiol Rev. 2013 Jul;26(3):526-46
pubmed: 23824372
Int J Antimicrob Agents. 2013 Feb;41(2):122-5
pubmed: 23158541
Clin Infect Dis. 2010 Jan 1;50 Suppl 1:S26-33
pubmed: 20067390
Clin Infect Dis. 2004 Jul 1;39(1):92-7
pubmed: 15206059
Mol Microbiol. 1994 Apr;12(1):105-14
pubmed: 8057831
Clin Infect Dis. 1996 Dec;23 Suppl 1:S73-7
pubmed: 8953110
Expert Rev Anti Infect Ther. 2007 Dec;5(6):991-1006
pubmed: 18039083
J Clin Microbiol. 2013 Mar;51(3):782-6
pubmed: 23254126
Antimicrob Resist Infect Control. 2019 Jun 14;8:99
pubmed: 31210928
Antimicrob Agents Chemother. 2008 Sep;52(9):3161-8
pubmed: 18625771
Antimicrob Agents Chemother. 2002 Sep;46(9):2908-13
pubmed: 12183246
Clin Microbiol Infect. 2011 Mar;17(3):371-9
pubmed: 20456453
Clin Infect Dis. 2014 Sep 1;59(5):698-705
pubmed: 24867792
J Clin Microbiol. 2017 Jul;55(7):1998-2008
pubmed: 28404671
Anaerobe. 2020 Aug;64:102215
pubmed: 32574601
Clin Microbiol Infect. 2003 Jun;9(6):475-88
pubmed: 12848722
Eur J Clin Microbiol Infect Dis. 2014 Nov;33(11):2041-52
pubmed: 24930042
Anaerobe. 2015 Apr;32:1-6
pubmed: 25460195
Infect Dis (Lond). 2019 Oct;51(10):779-781
pubmed: 31368384
Antibiotics (Basel). 2017 Nov 07;6(4):
pubmed: 29112122
Anaerobe. 2017 Feb;43:21-26
pubmed: 27867083
Anaerobe. 2019 Feb;55:40-53
pubmed: 30316817
Anaerobe. 2012 Feb;18(1):37-43
pubmed: 22261518
Eur J Microbiol Immunol (Bp). 2020 Jun 05;10(2):64-75
pubmed: 32590337
Clin Microbiol Infect. 2019 Sep;25(9):1156.e9-1156.e13
pubmed: 30802650
Anaerobe. 2017 Feb;43:69-74
pubmed: 27890724
J Antimicrob Chemother. 2006 Sep;58(3):705-6
pubmed: 16867996
Antimicrob Agents Chemother. 2005 Mar;49(3):1253-6
pubmed: 15728943
J Glob Antimicrob Resist. 2019 Mar;16:210-214
pubmed: 30342928