Direct contact between Plasmodium falciparum and human B-cells in a novel co-culture increases parasite growth and affects B-cell growth.
B-cell
Culture
Human
Malaria
Plasmodium falciparum
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
05 Jul 2021
05 Jul 2021
Historique:
received:
27
09
2020
accepted:
24
06
2021
entrez:
6
7
2021
pubmed:
7
7
2021
medline:
14
10
2021
Statut:
epublish
Résumé
Plasmodium falciparum parasites cause malaria and co-exist in humans together with B-cells for long periods of time. Immunity is only achieved after repeated exposure. There has been a lack of methods to mimic the in vivo co-occurrence, where cells and parasites can be grown together for many days, and it has been difficult with long time in vitro studies. A new method for growing P. falciparum in 5% CO These results demonstrate that P. falciparum and B-cells undoubtedly can affect each other when allowed to multiply together, which is valuable information for future vaccine studies.
Sections du résumé
BACKGROUND
BACKGROUND
Plasmodium falciparum parasites cause malaria and co-exist in humans together with B-cells for long periods of time. Immunity is only achieved after repeated exposure. There has been a lack of methods to mimic the in vivo co-occurrence, where cells and parasites can be grown together for many days, and it has been difficult with long time in vitro studies.
METHODS AND RESULTS
RESULTS
A new method for growing P. falciparum in 5% CO
CONCLUSIONS
CONCLUSIONS
These results demonstrate that P. falciparum and B-cells undoubtedly can affect each other when allowed to multiply together, which is valuable information for future vaccine studies.
Identifiants
pubmed: 34225761
doi: 10.1186/s12936-021-03831-x
pii: 10.1186/s12936-021-03831-x
pmc: PMC8256226
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
303Commentaires et corrections
Type : ErratumIn
Références
Murray CJL, Rosenfeld LC, Lim SS, Andrews KG, Foreman KJ, Haring D, et al. Global malaria mortality between 1980 and 2010: a systematic analysis. Lancet. 2012;379:413–31.
doi: 10.1016/S0140-6736(12)60034-8
Beeson JG, Osier FHA, Engwerda CR. Recent insights into humoral and cellular immune responses against malaria. Trends Parasitol. 2008;24:578–84.
doi: 10.1016/j.pt.2008.08.008
Dorfman JR, Bejon P, Ndungu FM, Langhorne J, Kortok MM, Lowe BS, et al. B-cell memory to 3 Plasmodium falciparum blood-stage antigens in a malaria-endemic area. J Infect Dis. 2005;191:1623–30.
doi: 10.1086/429671
Langhorne J, Ndungu FM, Sponaas A-M, Marsh K. Immunity to malaria: more questions than answers. Nat Immunol. 2008;9:725–32.
doi: 10.1038/ni.f.205
Struik SS, Riley EM. Does malaria suffer from lack of memory? Immunol Rev. 2004;201:268–90.
doi: 10.1111/j.0105-2896.2004.00181.x
Marsh K, Otoo L, Hayes RJ, Carson DC, Greenwood BM. Antibodies to blood stage antigens of Plasmodium falciparum in rural Gambians and their relation to protection against infection. Trans R Soc Trop Med Hyg. 1989;83:293–303.
doi: 10.1016/0035-9203(89)90478-1
Giha HA, Staalsoe T, Dodoo D, Elhassan IM, Roper C, Satti GMH, et al. Nine-year longitudinal study of antibodies to variant antigens on the surface of Plasmodium falciparum-infected erythrocytes. Infect Immun. 1999;67:4092–8.
doi: 10.1128/IAI.67.8.4092-4098.1999
Cavanagh DR, Elhassan IM, Roper C, Robinson VJ, Giha H, Holder AA, et al. A longitudinal study of type-specific antibody responses to Plasmodium falciparum merozoite surface protein-1 in an area of unstable malaria in Sudan. J Immunol. 1998;161:347–59.
pubmed: 9647243
pmcid: 9647243
Gupta S, Snow RW, Donnelly CA, Marsh K, Newbold C. Immunity to non-cerebral severe malaria is acquired after one or two infections. Nat Med. 1999;5:340–3.
doi: 10.1038/6560
Weidanz WP, Long CA. The role of T cells in immunity to malaria. Progr Allergy. 1988;41:215–52.
Cohen S, McGregor IA, Carrington S. Gamma-globulin and acquired immunity to human malaria. Nature. 1961;192:733–7.
doi: 10.1038/192733a0
Wipasa J, Elliott S, Xu H, Good MF. Immunity to asexual blood stage malaria and vaccine approaches. Immunol Cell Biol. 2002;80:401–14.
doi: 10.1046/j.1440-1711.2002.01107.x
Ly A, Hansen DS. Development of B-cell memory in malaria. Front Immunol. 2019;10:559.
doi: 10.3389/fimmu.2019.00559
Weiss GE, Crompton PD, Li S, Walsh LA, Moir S, Traore B, et al. Atypical memory B-cells are greatly expanded in individuals living in a malaria-endemic area. J Immunol. 2009;183:2176–82.
doi: 10.4049/jimmunol.0901297
Weiss GE, Traore B, Kayentao K, Ongoiba A, Doumbo S, Doumtabe D, et al. The Plasmodium falciparum-specific human memory B-cell compartment expands gradually with repeated malaria infections. PLoS Pathog. 2010;6: e1000912.
doi: 10.1371/journal.ppat.1000912
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, et al. Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T Cell exhaustion. J Immunol. 2013;190:1038–47.
doi: 10.4049/jimmunol.1202438
Weiss GE, Clark EH, Li S, Traore B, Kayentao K, Ongoiba A, et al. A positive correlation between atypical memory B-cells and Plasmodium falciparum transmission intensity in cross-sectional studies in Peru and Mali. PLoS ONE. 2011;6: e15983.
doi: 10.1371/journal.pone.0015983
Yeo KT, Embury P, Anderson T, Mungai P, Malhotra I, King C, et al. HIV, cytomegalovirus, and malaria infections during pregnancy lead to inflammation and shifts in memory B-cell subsets in Kenyan neonates. J Immunol. 2019;202:1465–78.
doi: 10.4049/jimmunol.1801024
Amu S, Ruffin N, Rethi B, Chiodi F. Impairment of B-cell functions during HIV-1 infection. AIDS. 2013;27:2323–34.
doi: 10.1097/QAD.0b013e328361a427
Kardava L, Moir S, Wang W, Ho J, Buckner CM, Posada JG, et al. Attenuation of HIV-associated human B-cell exhaustion by siRNA downregulation of inhibitory receptors. J Clin Invest. 2011;121:2614–24.
doi: 10.1172/JCI45685
Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O’Shea MA, et al. Evidence for HIV-associated B-cell exhaustion in a dysfunctional memory B-cell compartment in HIV-infected viremic individuals. J Exp Med. 2008;205:1797–805.
doi: 10.1084/jem.20072683
Trager W, Jensen J. Human malaria parasites in continuous culture. Science. 1976;193:673–5.
doi: 10.1126/science.781840
Ribacke U, Moll K, Albrecht L, Ismail HA, Normark J, Flaberg E, et al. Improved in vitro culture of Plasmodium falciparum permits establishment of clinical isolates with preserved multiplication, invasion and rosetting phenotypes. PLoS ONE. 2013;8: e69781.
doi: 10.1371/journal.pone.0069781
Rono J, Färnert A, Olsson D, Osier F, Rooth I, Persson KEM. Plasmodium falciparum line-dependent association of in vitro growth-inhibitory activity and risk of malaria. Infect Immun. 2012;80:1900–8.
doi: 10.1128/IAI.06190-11
Flaberg E, Sabelström P, Strandh C, Szekely L. Extended field laser confocal microscopy (EFLCM): combining automated Gigapixel image capture with in silicovirtual microscopy. BMC Med Imag. 2008;8:13.
doi: 10.1186/1471-2342-8-13
Crompton PD, Mircetic M, Weiss G, Baughman A, Huang C-Y, Topham DJ, et al. The TLR9 ligand CpG promotes the acquisition of Plasmodium falciparum-specific memory B-cells in malaria-naïve individuals. J Immunol. 2009;182:3318–26.
doi: 10.4049/jimmunol.0803596
Dobaño C, Santano R, Vidal M, Jiménez A, Jairoce C, Ubillos I, et al. Differential patterns of IgG subclass responses to Plasmodium falciparum antigens in relation to malaria protection and RTS,S vaccination. Front Immunol. 2019;10:439.
doi: 10.3389/fimmu.2019.00439
Jogdand GM, Sengupta S, Bhattacharya G, Singh SK, Barik PK, Devadas S. Inducible costimulator expressing T cells promote parasitic growth during blood stage Plasmodium berghei ANKA infection. Front Immunol. 2018;9:1041.
doi: 10.3389/fimmu.2018.01041
Struik SS, Omer FM, Artavanis-Tsakonas K, Riley EM. Uninfected erythrocytes inhibit Plasmodium falciparum-induced cellular immune responses in whole-blood assays. Blood. 2004;103:3084–92.
doi: 10.1182/blood-2003-08-2867
Donati D, Zhang LP, Chen Q, Chêne A, Flick K, Nyström M, et al. Identification of a polyclonal B-cell activator in Plasmodium falciparum. Infect Immun. 2004;72:5412–8.
doi: 10.1128/IAI.72.9.5412-5418.2004
Donati D, Mok B, Chêne A, Xu H, Thangarajh M, Glas R, et al. Increased B-cell survival and preferential activation of the memory compartment by a malaria polyclonal B-cell activator. J Immunol. 2006;177:3035–44.
doi: 10.4049/jimmunol.177.5.3035
Derkach A, Otim I, Pfeiffer RM, Onabajo OO, Legason ID, Nabalende H, et al. Associations between IgG reactivity to Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens and Burkitt lymphoma in Ghana and Uganda case-control studies. EBioMedicine. 2019;39:358–68.
doi: 10.1016/j.ebiom.2018.12.020
Muellenbeck MF, Ueberheide B, Amulic B, Epp A, Fenyo D, Busse CE, et al. Atypical and classical memory B-cells produce Plasmodium falciparum neutralizing antibodies. J Exp Med. 2013;210:389–99.
doi: 10.1084/jem.20121970
Murugan R, Buchauer L, Triller G, Kreschel C, Costa G, Pidelaserra Martí G, et al. Clonal selection drives protective memory B-cell responses in controlled human malaria infection. Sci Immunol. 2018;3: eaap8029.
doi: 10.1126/sciimmunol.aap8029
Capolunghi F, Cascioli S, Giorda E, Rosado MM, Plebani A, Auriti C, et al. CpG drives human transitional B-cells to terminal differentiation and production of natural antibodies. J Immunol. 2008;180:800–8.
doi: 10.4049/jimmunol.180.2.800
Siewe B, Nipper AJ, Sohn H, Stapleton JT, Landay A. FcRL4 expression identifies a pro-inflammatory B-cell Subset in viremic HIV-infected subjects. Frontiers Immunol. 2017;8:1339.
doi: 10.3389/fimmu.2017.01339
Jourdan M, Robert N, Cren M, Thibaut C, Duperray C, Kassambara A, et al. Characterization of human FCRL4-positive B-cells. PLoS ONE. 2017;12: e0179793.
doi: 10.1371/journal.pone.0179793
Lugaajju A, Reddy SB, Wahlgren M, Kironde F, Persson KEM. Development of Plasmodium falciparum specific naïve, atypical, memory and plasma B-cells during infancy and in adults in an endemic area. Malar J. 2017;16:37.
doi: 10.1186/s12936-017-1697-z