Mast cell activation tests by flow cytometry: A new diagnostic asset?
Journal
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
ISSN: 1365-2222
Titre abrégé: Clin Exp Allergy
Pays: England
ID NLM: 8906443
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
22
04
2021
accepted:
04
07
2021
pubmed:
8
7
2021
medline:
1
4
2022
entrez:
7
7
2021
Statut:
ppublish
Résumé
Since the late nineties, evidence has accumulated that flow-assisted basophil activation test (BAT) might be an accessible and reliable method to explore the mechanisms governing basophil degranulation and diagnostic allowing correct prediction of the clinical outcome following exposure to the offending allergen(s) and cross-reactive structures for different IgE-dependent allergies and particular forms of autoimmune urticaria. Although the BAT offers many advantages over mediator release tests, it is left with some weaknesses that hinder a wider application. It is preferable to perform the BAT analysis within 4 h of collection, and the technique does not advance diagnosis in patients with non-responsive cells. Besides, the BAT is difficult to standardize mainly because of the difficulty to perform large batch analyses that might span over several days. This article reviews the status of flow cytometric mast cell activation test (MAT) using passively sensitized mast cells (MCs) with patients' sera or plasma (henceforth indicated as passive MAT; pMAT) using both MC lines and cultured MCs in the diagnosis of IgE-dependent allergies. In addition, this paper provides guidance for generating human MCs from peripheral blood CD34
Substances chimiques
MRGPRX2 protein, human
0
Nerve Tissue Proteins
0
Receptors, G-Protein-Coupled
0
Receptors, Neuropeptide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1482-1500Subventions
Organisme : Fonds Wetenschappelijk Onderzoek
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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