Acceleration of age-induced proteolysis in the guinea pig lens nucleus by in vivo exposure to hyperbaric oxygen: A mass spectrometry analysis.

Aging / physiology Animals Aquaporins / metabolism Cataract / etiology Chromatography, Liquid Crystallins / metabolism Cytoskeletal Proteins / metabolism Disease Models, Animal Eye Proteins / metabolism Guinea Pigs Hyperbaric Oxygenation / adverse effects Lens Nucleus, Crystalline / metabolism Proteolysis / drug effects Tandem Mass Spectrometry alpha-Crystallin A Chain / metabolism
Alpha crystallin Aquaporin-0 Hyperbaric oxygen Lens Mass spectrometry Nuclear cataract Protein truncation αA66-80 peptide

Journal

Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707

Informations de publication

Date de publication:
09 2021
Historique:
received: 31 03 2021
revised: 21 06 2021
accepted: 30 06 2021
pubmed: 8 7 2021
medline: 9 10 2021
entrez: 7 7 2021
Statut: ppublish

Résumé

Hyperbaric oxygen (HBO) treatment of animals or ocular lenses in culture recapitulates many molecular changes observed in human age-related nuclear cataract. The guinea pig HBO model has been one of the best examples of such treatment leading to dose-dependent development of lens nuclear opacities. In this study, complimentary mass spectrometry methods were employed to examine protein truncation after HBO treatment of aged guinea pigs. Quantitative liquid chromatography-mass spectrometry (LC-MS) analysis of the membrane fraction of guinea pig lenses showed statistically significant increases in aquaporin-0 (AQP0) C-terminal truncation, consistent with previous reports of accelerated loss of membrane and cytoskeletal proteins. In addition, imaging mass spectrometry (IMS) analysis spatially mapped the acceleration of age-related αA-crystallin truncation in the lens nucleus. The truncation sites in αA-crystallin closely match those observed in human lenses with age. Taken together, our results suggest that HBO accelerates the normal lens aging process and leads to nuclear cataract.

Identifiants

DOI: 10.1016/j.exer.2021.108697 PMID: 34233175 PMC: PMC8429224
pubmed: 34233175
pii: S0014-4835(21)00263-3
doi: 10.1016/j.exer.2021.108697
pmc: PMC8429224
mid: NIHMS1723366
pii:
doi:

Substances chimiques

Aquaporins 0
Crystallins 0
Cytoskeletal Proteins 0
Eye Proteins 0
alpha-Crystallin A Chain 0
aquaporin 0 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

108697

Subventions

Organisme : NEI NIH HHS
ID : R01 EY024258
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY013462
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY008126
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY002027
Pays : United States
Organisme : NEI NIH HHS
ID : U54 EY032442
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

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Auteurs

Frank J Giblin (FJ)

Eye Research Institute, Oakland University, Rochester, MI, 48309, USA.

David M G Anderson (DMG)

Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA.

Jun Han (J)

Genome BC Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada.

Kristie L Rose (KL)

Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA.

Zhen Wang (Z)

Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA.

Kevin L Schey (KL)

Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37242, USA. Electronic address: kevin.schey@vanderbilt.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Vieillissement › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Eucaryotes › Animaux › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Porines › Aquaporines › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Maladies de l'oeil › Maladies du cristallin › Cataracte › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de l'oeil › Cristallines › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de l'oeil › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Cochons d'Inde › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Thérapeutique › Thérapie respiratoire › Oxygénothérapie › Oxygénation hyperbare › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Organes des sens › Oeil › Pôle antérieur du bulbe oculaire › Cristallin › Noyau du cristallin › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Métabolisme › Protéolyse › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Acceleration of age-induced proteolysis in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Cristallines alpha › Chaine A de la cristalline alpha › Acceleration of age-induced proteolysis in the...