Distinct Roles of Perilipins in the Intramuscular Deposition of Lipids in Glutamine-Supplemented, Low-, and Normal-Birth-Weight Piglets.
glutamine
lipid deposition
low birth weight
muscle gene expression
perilipins
pig
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
11
2020
accepted:
10
05
2021
entrez:
8
7
2021
pubmed:
9
7
2021
medline:
9
7
2021
Statut:
epublish
Résumé
Piglets with low birth weight (LBW) usually have reduced muscle mass and increased lipid deposition compared with their normal-birth-weight (NBW) littermates. Supplementation of piglets with amino acids during the first days of life may improve muscle growth and simultaneously alter the intramuscular lipid deposition. The aim of the current study was to investigate the influence of glutamine (Gln) supplementation during the early suckling period on lipid deposition in the longissimus muscle (MLD) and the role of different perilipin (PLIN) family members in this process. Four groups were generated consisting of 72 male LBW piglets and 72 NBW littermates. Piglets were supplemented with either 1 g Gln/kg body weight or an isonitrogenous amount of alanine (Ala) between days post natum (dpn) 1 and 12. Twelve piglets per group were slaughtered at 5, 12, and 26 dpn, and muscle tissue was collected. Perilipins were localized by immunohistochemistry in muscle sections. The mRNA and protein abundances of PLIN family members and related lipases were quantified by quantitative RT-PCR (qPCR) and western blots, respectively. While PLIN1 was localized around lipid droplets in mature and developing adipocytes, PLIN2 was localized at intramyocellular lipid droplets, PLIN3 and 4 at cell membranes of muscle fibers and adipocytes, and PLIN5 in the cytoplasm of undefined cells. The western blot results indicated higher protein abundances of PLIN2, 3, 4, and 5 in LBW piglets (
Identifiants
pubmed: 34235195
doi: 10.3389/fvets.2021.633898
pmc: PMC8257002
doi:
Types de publication
Journal Article
Langues
eng
Pagination
633898Informations de copyright
Copyright © 2021 Zhao, Albrecht, Li, Schregel, Sciascia, Metges and Maak.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Clin Invest. 1995 Aug;96(2):976-86
pubmed: 7635990
Sci Rep. 2016 Sep 16;6:33362
pubmed: 27634466
Histochem Cell Biol. 2009 May;131(5):575-81
pubmed: 19169702
Curr Opin Cell Biol. 2008 Aug;20(4):378-85
pubmed: 18606534
Biochim Biophys Acta. 2013 Apr;1831(4):844-52
pubmed: 23353597
Am J Physiol Endocrinol Metab. 2009 Aug;297(2):E271-88
pubmed: 19318514
J Biol Chem. 2011 Feb 18;286(7):5126-35
pubmed: 21148142
J Biol Chem. 2011 May 6;286(18):15707-15
pubmed: 21393244
Nat Rev Mol Cell Biol. 2013 Dec;14(12):775-86
pubmed: 24220094
J Biol Chem. 2012 Jan 20;287(4):2273-9
pubmed: 22090029
Diabetes. 2006 Dec;55(12):3418-28
pubmed: 17130488
Genes (Basel). 2018 Apr 04;9(4):
pubmed: 29617344
J Biol Chem. 2009 Jan 30;284(5):3049-3057
pubmed: 19064991
Biochim Biophys Acta. 2014 Jul;1841(7):919-33
pubmed: 24721265
J Nutr Metab. 2012;2012:545341
pubmed: 22315674
Mol Biol Cell. 2005 Apr;16(4):2091-105
pubmed: 15689493
J Anim Sci. 2011 Jul;89(7):2017-30
pubmed: 21169511
Lipids Health Dis. 2017 Apr 28;16(1):83
pubmed: 28454542
Histochem Cell Biol. 2012 Feb;137(2):205-16
pubmed: 22127648
Animal. 2010 Feb;4(2):303-19
pubmed: 22443885
Cell Metab. 2015 Nov 3;22(5):851-60
pubmed: 26411340
Malawi Med J. 2012 Sep;24(3):69-71
pubmed: 23638278
Curr Opin Lipidol. 2008 Oct;19(5):441-7
pubmed: 18769224
Amino Acids. 2009 May;37(1):1-17
pubmed: 19301095
Lipids. 2009 Nov;44(11):1029-37
pubmed: 19847466
Trends Biochem Sci. 1999 Mar;24(3):109-15
pubmed: 10203758
J Biol Chem. 2009 Nov 6;284(45):30941-8
pubmed: 19748893
Am J Physiol Regul Integr Comp Physiol. 2013 Apr 15;304(8):R644-50
pubmed: 23408028
Int J Biol Sci. 2017 Apr 10;13(5):532-544
pubmed: 28539828
J Biol Chem. 2001 Feb 16;276(7):5101-8
pubmed: 11084026
Diabetes. 2004 May;53(5):1243-52
pubmed: 15111493
Am J Physiol Endocrinol Metab. 2002 Oct;283(4):E775-83
pubmed: 12217895
Genome Biol. 2007;8(2):R19
pubmed: 17291332
J Cell Biol. 2009 May 18;185(4):641-55
pubmed: 19451273
J Diabetes Res. 2017;2017:1789395
pubmed: 28676863
Neonatology. 2011;99(3):208-16
pubmed: 20881437
J Genomics. 2013 Nov 08;1:29-38
pubmed: 25031653
J Lipid Res. 1995 Jun;36(6):1211-26
pubmed: 7665999
Front Physiol. 2018 Mar 06;9:172
pubmed: 29559925
J Biol Chem. 1999 Jun 11;274(24):16825-30
pubmed: 10358026
BMC Res Notes. 2011 Oct 24;4:441
pubmed: 22023805
J Biol Chem. 1991 Jun 15;266(17):11341-6
pubmed: 2040638
J Anim Sci. 2006 Jan;84(1):93-103
pubmed: 16361495
Meat Sci. 2014 Jan;96(1):157-64
pubmed: 23896150
J Biol Chem. 2003 Sep 26;278(39):37713-21
pubmed: 12840023
Annu Rev Biochem. 2012;81:687-714
pubmed: 22524315
J Biol Chem. 2000 Dec 8;275(49):38486-93
pubmed: 10948207
J Biol Chem. 2005 Dec 30;280(52):42841-7
pubmed: 16115879
BMC Biotechnol. 2006 Oct 09;6:41
pubmed: 17026777
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7856-60
pubmed: 1518805
J Lipid Res. 2007 Dec;48(12):2751-61
pubmed: 17872589
Animals (Basel). 2020 Oct 28;10(11):
pubmed: 33126436
Apoptosis. 2009 Dec;14(12):1484-95
pubmed: 19421860
Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7522-7
pubmed: 11390966
Biochim Biophys Acta. 2007 Feb;1771(2):210-27
pubmed: 17234449
Mamm Genome. 2001 Sep;12(9):741-9
pubmed: 11641724
Adipocyte. 2014 Dec 10;3(4):242-55
pubmed: 26317048
Adipocyte. 2013 Apr 1;2(2):80-6
pubmed: 23805403
J Biol Chem. 2006 May 19;281(20):14232-40
pubmed: 16571721
J Lipid Res. 1997 Nov;38(11):2249-63
pubmed: 9392423
Appl Physiol Nutr Metab. 2015 Jul;40(7):641-51
pubmed: 25971423
Meat Sci. 2011 Aug;88(4):631-7
pubmed: 21420243
J Lipid Res. 1977 May;18(3):363-70
pubmed: 16978
Arch Physiol Biochem. 2012 Feb;118(1):22-30
pubmed: 22117101
J Lipid Res. 2011 Dec;52(12):2159-2168
pubmed: 21885430
Am J Physiol Regul Integr Comp Physiol. 2012 Jan 1;302(1):R29-36
pubmed: 22012700
Semin Cell Dev Biol. 1999 Feb;10(1):51-8
pubmed: 10355028
J Biol Chem. 2005 May 13;280(19):19146-55
pubmed: 15731108
J Lipid Res. 2010 Mar;51(3):468-71
pubmed: 19638644
Physiol Rep. 2015 Aug;3(8):
pubmed: 26265748
J Lipid Res. 2009 Jan;50(1):3-21
pubmed: 18952573
J Biol Chem. 2004 Jul 16;279(29):30490-7
pubmed: 15136565