The allosteric control mechanism of bacterial glycogen biosynthesis disclosed by cryoEM.
AGPase, ADP-glucose pyrophosphorylase
AMP, adenosine 5′-monophosphate
ATP, adenosine 5′-triphosphate
EcAGPase, AGPase from E. coli
Enzyme allosterism
FBP, fructose 1,6-bisphosphate
G1P, α-d-glucose-1-phosphate
GBE, glycogen branching enzyme
GDE, glycogen debranching enzyme
GP, glycogen phosphorylase
GS, glycogen synthase
GTA-like, glycosyltransferase-A like domain
Glycogen biosynthesis
Glycogen regulation
LβH, left-handed β-helix domain
Nucleotide sugar biosynthesis
PPi, pyrophosphate
RIN, residue interaction network
SM, sensory motif
Journal
Current research in structural biology
ISSN: 2665-928X
Titre abrégé: Curr Res Struct Biol
Pays: Netherlands
ID NLM: 101767537
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
02
2020
revised:
12
04
2020
accepted:
20
04
2020
entrez:
8
7
2021
pubmed:
1
5
2020
medline:
1
5
2020
Statut:
epublish
Résumé
Glycogen and starch are the major carbon and energy reserve polysaccharides in nature, providing living organisms with a survival advantage. The evolution of the enzymatic machinery responsible for the biosynthesis and degradation of such polysaccharides, led the development of mechanisms to control the assembly and disassembly rate, to store and recover glucose according to cell energy demands. The tetrameric enzyme ADP-glucose pyrophosphorylase (AGPase) catalyzes and regulates the initial step in the biosynthesis of both α-polyglucans. AGPase displays cooperativity and allosteric regulation by sensing metabolites from the cell energy flux. The understanding of the allosteric signal transduction mechanisms in AGPase arises as a long-standing challenge. In this work, we disclose the cryoEM structures of the paradigmatic homotetrameric AGPase from
Identifiants
pubmed: 34235472
doi: 10.1016/j.crstbi.2020.04.005
pii: S2665-928X(20)30009-X
pmc: PMC8244506
doi:
Types de publication
Journal Article
Langues
eng
Pagination
89-103Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no financial conflict of interest.
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