The gut brain in a dish: Murine primary enteric nervous system cell cultures.
adult mouse
enteric glial cells
enteric neurons
primary ENS culture
protocol
Journal
Neurogastroenterology and motility
ISSN: 1365-2982
Titre abrégé: Neurogastroenterol Motil
Pays: England
ID NLM: 9432572
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
22
05
2021
received:
29
01
2021
accepted:
01
06
2021
pubmed:
9
7
2021
medline:
5
4
2022
entrez:
8
7
2021
Statut:
ppublish
Résumé
The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
Sections du résumé
BACKGROUND
The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease.
PURPOSE
This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
Identifiants
pubmed: 34236124
doi: 10.1111/nmo.14215
pmc: PMC9285479
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14215Informations de copyright
© 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd.
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