Extracellular Flux Analysis to Investigate the Impact of NF-κB on Mitochondrial Respiration in Colorectal Carcinoma (CRC).
Cancer
Colorectal carcinoma
Extracellular Flux Analysis
Metabolism
NF-κB
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
8
7
2021
pubmed:
9
7
2021
medline:
11
1
2022
Statut:
ppublish
Résumé
The reprogramming of cell metabolism is a hallmark of cancer. NF-κB transcription factors coordinate the host defense responses to stress, injury, and infection. They also play a central role in oncogenesis, at least in part by regulating cell metabolism and the adaptation to energy stress conditions in various types of cancer, such as colorectal carcinoma (CRC). Here, we describe the XF Cell Mito Stress Test methodology aimed at characterizing the metabolic and bioenergetic profile of CRC cells following the silencing of the essential NF-κB subunit, RelA. This methodology may also be applied to other cancers to reveal novel core vulnerabilities of malignant cells.
Identifiants
pubmed: 34236646
doi: 10.1007/978-1-0716-1669-7_18
doi:
Substances chimiques
NF-kappa B
0
Transcription Factor RelA
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
293-303Subventions
Organisme : Medical Research Council
ID : MR/L005069/1
Pays : United Kingdom
Organisme : Department of Health
ID : WIIS_P81135
Pays : United Kingdom
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