Conditional Knockout Mouse Models to Study the Roles of Individual NF-κB Transcription Factors in Lymphocytes.
Conditional alleles
Cre/loxP
NF-κB
NF-κB subunits
NF-κB transcription factors
Nuclear factor-κB
Transgenic animal models
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
8
7
2021
pubmed:
9
7
2021
medline:
11
1
2022
Statut:
ppublish
Résumé
The NF-κB signal transduction pathway has crucial functions in cell growth, survival, and the development of lymphocytes and other immune cells. Upon activation of the pathway, five distinct NF-κB transcription factor subunits that occur as homodimers or heterodimers comprise the downstream mediators that transcribe NF-κB target genes. A major quest in NF-κB research is to understand the biology of the separate subunits. However, determining the functions of the individual subunits using constitutional knockout mice is often hampered by the marked cell type and/or developmental stage-specific activation of the NF-κB pathway. To overcome these problems, we and others have generated loxP-flanked alleles of the genes encoding the different NF-κB subunits that upon crossing to suitable Cre-expressing mouse lines can be conditionally deleted in the desired cell type or developmental stage. We here describe the basic characteristics of conditional NF-κB subunit alleles rel (encoding c-REL), rela (RELA), relb (RELB), and nfkb2 (NF-κB2) generated in our laboratory that are available to the research community through a repository, and provide basic methods to study the consequences of tissue-specific ablation of NF-κB transcription factors in lymphocytes.
Identifiants
pubmed: 34236647
doi: 10.1007/978-1-0716-1669-7_19
doi:
Substances chimiques
NF-kappa B
0
Transcription Factor RelA
0
Transcription Factor RelB
147337-75-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
305-319Références
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