Functional analysis of zona pellucida domain protein Dusky in Tribolium castaneum.
20-hydroxyecdysone
4E-BP
Dusky
Tribolium castaneum
wing
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
revised:
20
04
2021
received:
04
03
2021
accepted:
07
05
2021
pubmed:
9
7
2021
medline:
1
4
2022
entrez:
8
7
2021
Statut:
ppublish
Résumé
The zona pellucida domain protein Dusky (Dy) plays a vital role in wing morphogenesis in insects, but little information on its function has been reported. In this study, we found that dy regulated wing cell size, larval and pupal duration, and the metabolism of amino acid and 20-hydroxyecdysone in Tribolium castaneum. Using RNA-seq, 413 differentially expressed genes were identified between physiological buffer-injected and dy-double-stranded RNA-treated larvae, including 88 downregulated genes and 325 upregulated genes. Among these genes, dy knockdown increased CYP18A1 expression to elevate the 26-hydroxylation of 20-hydroxyecdysone, which ultimately led to growth defects in wing cells. Silencing of dy upregulated the transcription of genes encoding tyrosine aminotransferase, 4-hydroxyphenylpyruvate dioxygenase, homogentisate 1, 2-dioxygenase, and Pale to promote the catabolism of tyrosine and phenylalanine, which eventually reduced amino acid content. Furthermore, dy knockdown upregulated 4E-BP expression, and 4E-BP silencing partially phenocopied dy RNA interference-mediated wing morphogenesis. These results suggest that Dy controls 20-hydroxyecdysone and amino acid metabolism to regulate wing morphogenesis in the insect.
Identifiants
pubmed: 34237197
doi: 10.1111/1744-7917.12938
doi:
Substances chimiques
Insect Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
388-398Subventions
Organisme : National Natural Science Foundation of China
ID : 32070501
Organisme : National Natural Science Foundation of China
ID : 31601893
Informations de copyright
© 2021 Institute of Zoology, Chinese Academy of Sciences.
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