Consequences of the different order of immunoglobulin gene rearrangements in swine.


Journal

Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205

Informations de publication

Date de publication:
01 2022
Historique:
received: 23 04 2021
revised: 14 06 2021
accepted: 30 06 2021
pubmed: 10 7 2021
medline: 8 4 2022
entrez: 9 7 2021
Statut: ppublish

Résumé

Swine use a reverse order of immunoglobulin chain rearrangement compared to humans and mice, and this altered and modified order should have measurable consequences. Here we perform new and defining experiments with developing and mature B cells, characterizing the B cell populations that do not exist in other species. First, we have finally confirmed that light chains κ and λ are rearranged and expressed on the surface before any heavy chain rearrangements using western-blot. And second, we have analyzed a pool of mature B cells on the single-cell level to demonstrate that many κ

Identifiants

pubmed: 34242678
pii: S0145-305X(21)00204-4
doi: 10.1016/j.dci.2021.104196
pii:
doi:

Substances chimiques

Immunoglobulin Light Chains 0
Immunoglobulin kappa-Chains 0
Immunoglobulin lambda-Chains 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104196

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Marek Sinkora (M)

Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic. Electronic address: marek@biomed.cas.cz.

Katerina Stepanova (K)

Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic.

Jana Sinkorova (J)

Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic.

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Classifications MeSH