Abundant TNF-LIGHT expression in the airways of patients with asthma with persistent airflow limitation: Association with nitrative and inflammatory profiles.
Airway remodeling
Asthma
Inflammatory mediator
LIGHT
Nitrative stress
Journal
Respiratory investigation
ISSN: 2212-5353
Titre abrégé: Respir Investig
Pays: Netherlands
ID NLM: 101581124
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
12
01
2021
revised:
12
05
2021
accepted:
22
05
2021
pubmed:
11
7
2021
medline:
26
10
2021
entrez:
10
7
2021
Statut:
ppublish
Résumé
The role of the inflammatory secretory protein TNF-LIGHT (LIGHT) in the molecular mechanisms underlying persistent airflow limitation (PAL) in asthma remains unclear. We hypothesized that high airway LIGHT expression may be a feature of asthma with PAL associated with specific expression patterns of inflammatory molecules. This hypothesis was tested in 16 patients with asthma on inhaled corticosteroid treatment. Induced sputum was collected, the expression of LIGHT and 3-nitrotyrosine (NT), which reflects the footprint of reactive nitrogen species content, was measured using immunohistochemical staining, and the inflammatory molecules in the sputum supernatant were analyzed using a magnetic bead array. LIGHT staining in the cells had a significantly higher intensity in participants with PAL than in participants without PAL (47.9 × 10 The findings suggest that LIGHT is a key component in the association between airway inflammation and airflow limitation in patients with asthma, and its expression may be persistently correlated with the abundance of inflammatory cells and inflammatory and profibrogenic radical/molecules.
Sections du résumé
BACKGROUND
BACKGROUND
The role of the inflammatory secretory protein TNF-LIGHT (LIGHT) in the molecular mechanisms underlying persistent airflow limitation (PAL) in asthma remains unclear. We hypothesized that high airway LIGHT expression may be a feature of asthma with PAL associated with specific expression patterns of inflammatory molecules.
METHODS
METHODS
This hypothesis was tested in 16 patients with asthma on inhaled corticosteroid treatment. Induced sputum was collected, the expression of LIGHT and 3-nitrotyrosine (NT), which reflects the footprint of reactive nitrogen species content, was measured using immunohistochemical staining, and the inflammatory molecules in the sputum supernatant were analyzed using a magnetic bead array.
RESULTS
RESULTS
LIGHT staining in the cells had a significantly higher intensity in participants with PAL than in participants without PAL (47.9 × 10
CONCLUSIONS
CONCLUSIONS
The findings suggest that LIGHT is a key component in the association between airway inflammation and airflow limitation in patients with asthma, and its expression may be persistently correlated with the abundance of inflammatory cells and inflammatory and profibrogenic radical/molecules.
Identifiants
pubmed: 34244107
pii: S2212-5345(21)00106-4
doi: 10.1016/j.resinv.2021.05.011
pii:
doi:
Substances chimiques
TNFSF14 protein, human
0
Tumor Necrosis Factor Ligand Superfamily Member 14
0
Matrix Metalloproteinase 2
EC 3.4.24.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
651-660Informations de copyright
Copyright © 2021 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of Interest The authors have no conflicts of interest.