isoSTED microscopy with water-immersion lenses and background reduction.


Journal

Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626

Informations de publication

Date de publication:
17 08 2021
Historique:
received: 02 09 2020
revised: 26 04 2021
accepted: 18 05 2021
pubmed: 12 7 2021
medline: 11 9 2021
entrez: 11 7 2021
Statut: ppublish

Résumé

Fluorescence microscopy is an excellent tool to gain knowledge on cellular structures and biochemical processes. Stimulated emission depletion (STED) microscopy provides a resolution in the range of a few 10 nm at relatively fast data acquisition. As cellular structures can be oriented in any direction, it is of great benefit if the microscope exhibits an isotropic resolution. Here, we present an isoSTED microscope that utilizes water-immersion objective lenses and enables imaging of cellular structures with an isotropic resolution of better than 60 nm in living samples at room temperature and without CO

Identifiants

pubmed: 34246627
pii: S0006-3495(21)00556-7
doi: 10.1016/j.bpj.2021.05.031
pmc: PMC8392127
pii:
doi:

Substances chimiques

Water 059QF0KO0R

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3303-3314

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Auteurs

René Siegmund (R)

Department of Optical Nanoscopy, Institute for Nanophotonics Göttingen, Göttingen, Germany.

Frank Werner (F)

Institute of Mathematics, University of Würzburg, Würzburg, Germany.

Stefan Jakobs (S)

Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, Germany.

Claudia Geisler (C)

Department of Optical Nanoscopy, Institute for Nanophotonics Göttingen, Göttingen, Germany.

Alexander Egner (A)

Department of Optical Nanoscopy, Institute for Nanophotonics Göttingen, Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, Germany. Electronic address: alexander.egner@ifnano.de.

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Classifications MeSH