The Evolutionary Patterns of Genome Size in Ensifera (Insecta: Orthoptera).
C-value
Ensifera
flow cytometry
genome size
phylogeny
Journal
Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621
Informations de publication
Date de publication:
2021
2021
Historique:
received:
11
04
2021
accepted:
25
05
2021
entrez:
12
7
2021
pubmed:
13
7
2021
medline:
13
7
2021
Statut:
epublish
Résumé
Genomic size variation has long been a focus for biologists. However, due to the lack of genome size data, the mechanisms behind this variation and the biological significance of insect genome size are rarely studied systematically. The detailed taxonomy and phylogeny of the Ensifera, as well as the extensive documentation concerning their morphological, ecological, behavioral, and distributional characteristics, make them a strong model for studying the important scientific problem of genome size variation. However, data on the genome size of Ensifera are rather sparse. In our study, we used flow cytometry to determine the genome size of 32 species of Ensifera, the smallest one being only 1C = 0.952 pg with the largest species up to 1C = 19.135 pg, representing a 20-fold range. This provides a broader blueprint for the genome size variation of Orthoptera than was previously available. We also completed the assembly of nine mitochondrial genomes and combined mitochondrial genome data from public databases to construct phylogenetic trees containing 32 species of Ensifera and three outgroups. Based on these inferred phylogenetic trees, we detected the phylogenetic signal of genome size variation in Ensifera and found that it was strong in both males and females. Phylogenetic comparative analyses revealed that there were no correlations between genome size and body size or flight ability in Tettigoniidae. Reconstruction of ancestral genome size revealed that the genome size of Ensifera evolved in a complex pattern, in which the genome size of the grylloid clade tended to decrease while that of the non-grylloid clade expanded significantly albeit with fluctuations. However, the evolutionary mechanisms underlying variation of genome size in Ensifera are still unknown.
Identifiants
pubmed: 34249107
doi: 10.3389/fgene.2021.693541
pmc: PMC8261143
doi:
Types de publication
Journal Article
Langues
eng
Pagination
693541Informations de copyright
Copyright © 2021 Yuan, Huang, Mao, Zhang, Nie, Zhang, Zhou and Mao.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Cell Sci. 1978 Dec;34:247-78
pubmed: 372199
J Gen Physiol. 1951 Mar 20;34(4):451-62
pubmed: 14824511
Genome. 2000 Oct;43(5):750-5
pubmed: 11081963
Ecol Evol. 2017 Jun 22;7(15):5939-5947
pubmed: 28811889
J Evol Biol. 2017 Aug;30(8):1592-1608
pubmed: 28609564
Genome Res. 2000 Jul;10(7):908-15
pubmed: 10899140
Cytometry A. 2003 Feb;51(2):127-8; author reply 129
pubmed: 12541287
Nature. 2007 Mar 8;446(7132):180-4
pubmed: 17344851
J Mol Evol. 2017 Dec;85(5-6):169-171
pubmed: 29110026
Genome Res. 1999 Apr;9(4):317-24
pubmed: 10207154
Virus Evol. 2018 Jun 08;4(1):vey016
pubmed: 29942656
Evolution. 2003 Apr;57(4):717-45
pubmed: 12778543
Methods Mol Biol. 2011;772:3-12
pubmed: 22065429
Proc Natl Acad Sci U S A. 1950 Nov;36(11):643-54
pubmed: 14808154
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14080-5
pubmed: 14615584
Genome. 2000 Oct;43(5):895-901
pubmed: 11081981
Biol Rev Camb Philos Soc. 2001 Feb;76(1):65-101
pubmed: 11325054
Chromosoma. 2018 Sep;127(3):323-340
pubmed: 29549528
Front Physiol. 2020 Oct 22;11:567125
pubmed: 33192564
Chromosome Res. 2011 Aug;19(6):809-23
pubmed: 21877225
F1000Res. 2020 Jul 27;9:775
pubmed: 33163158
Bioinformatics. 2013 Oct 1;29(19):2487-9
pubmed: 23842809
Syst Biol. 2004 Oct;53(5):673-84
pubmed: 15545248
Cytometry A. 2010 Jul;77(7):635-42
pubmed: 20583277
Bioinformatics. 2014 May 1;30(9):1312-3
pubmed: 24451623
Genome Res. 2004 May;14(5):988-95
pubmed: 15123596
J Evol Biol. 2013 Oct;26(10):2171-83
pubmed: 23937477
Chromosoma. 2015 Jun;124(2):263-75
pubmed: 25472934
Mol Ecol Resour. 2021 Jan;21(1):226-237
pubmed: 32780934
Chromosome Res. 2011 Oct;19(7):925-38
pubmed: 22042526
Trends Genet. 2001 Jan;17(1):23-8
pubmed: 11163918
New Phytol. 2016 Apr;210(2):709-16
pubmed: 26595257
Nature. 1999 Oct 28;401(6756):877-84
pubmed: 10553904
Nucleic Acids Res. 2012 Apr;40(7):2833-45
pubmed: 22139921
Plant Mol Biol. 2003 Mar;51(4):523-31
pubmed: 12650618
Heredity (Edinb). 2005 Mar;94(3):280-94
pubmed: 15674378
Genome Biol Evol. 2017 Jun 1;9(6):1499-1512
pubmed: 28541478
Evol Biol. 2010 Dec;37(4):227-233
pubmed: 22140283
Bioinformatics. 2010 Aug 1;26(15):1907-9
pubmed: 20525823
PLoS One. 2017 Mar 7;12(3):e0173505
pubmed: 28267812
Genome Biol Evol. 2020 Jul 1;12(7):1180-1193
pubmed: 32539114
Nat Commun. 2014;5:2957
pubmed: 24423660
Syst Biol. 2012 May;61(3):539-42
pubmed: 22357727
Proc Natl Acad Sci U S A. 2018 May 1;115(18):4713-4718
pubmed: 29674453
Philos Trans R Soc Lond B Biol Sci. 1977 Mar 21;277(955):201-26
pubmed: 16285
Ann Bot. 2005 Jan;95(1):255-60
pubmed: 15596473
Mol Phylogenet Evol. 2017 Mar;108:22-33
pubmed: 28188878
Nat Rev Genet. 2005 Sep;6(9):699-708
pubmed: 16151375
Nucleic Acids Res. 2019 Jun 20;47(11):e63
pubmed: 30864657
Am J Bot. 2004 Oct;91(10):1709-25
pubmed: 21652319
Evolution. 2002 Jan;56(1):121-30
pubmed: 11913657
Genetica. 2002 May;115(1):131-46
pubmed: 12188045
Genetics. 1998 Jan;148(1):435-43
pubmed: 9475753
Plant Cell. 1999 Sep;11(9):1769-1784
pubmed: 10488242
BMC Genomics. 2021 Apr 29;22(1):311
pubmed: 33926388
Hereditas. 2003;139(2):121-7
pubmed: 15061813
Ann Bot. 2005 Jan;95(1):133-46
pubmed: 15596463
Biology (Basel). 2021 Mar 26;10(4):
pubmed: 33810583
BMC Biol. 2006 Dec 07;4:41
pubmed: 17156431
Genome. 2003 Aug;46(4):683-706
pubmed: 12897876
Mol Biol Evol. 2012 Jun;29(6):1695-701
pubmed: 22319168
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Evolution. 2014 Dec;68(12):3629-35
pubmed: 25200798
J Exp Biol. 2021 Apr 15;224(8):
pubmed: 33914029
Proc Biol Sci. 2009 Nov 7;276(1674):3753-7
pubmed: 19656792
Mitochondrial DNA A DNA Mapp Seq Anal. 2019 Apr;30(3):385-396
pubmed: 30289002
Bioinformatics. 2014 Jun 15;30(12):1660-6
pubmed: 24532719
Philos Trans R Soc Lond B Biol Sci. 2020 Mar 2;375(1793):20190146
pubmed: 31928192
J Cell Sci. 1983 Sep;63:135-46
pubmed: 6630307
Bioinformatics. 2013 Nov 15;29(22):2933-5
pubmed: 24008419