Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Bar-seq
S. pombe
cellular ageing
competitive growth
lifespan mutants
longevity
parallel genetic screen
Journal
Microbial cell (Graz, Austria)
ISSN: 2311-2638
Titre abrégé: Microb Cell
Pays: Austria
ID NLM: 101632887
Informations de publication
Date de publication:
05 Jul 2021
05 Jul 2021
Historique:
received:
03
03
2021
revised:
20
04
2021
accepted:
26
04
2021
entrez:
12
7
2021
pubmed:
13
7
2021
medline:
13
7
2021
Statut:
epublish
Résumé
Ageing-related processes are largely conserved, with simple organisms remaining the main platform to discover and dissect new ageing-associated genes. Yeasts provide potent model systems to study cellular ageing owing their amenability to systematic functional assays under controlled conditions. Even with yeast cells, however, ageing assays can be laborious and resource-intensive. Here we present improved experimental and computational methods to study chronological lifespan in
Identifiants
pubmed: 34250083
doi: 10.15698/mic2021.07.754
pii: MIC0271E115
pmc: PMC8246024
doi:
Types de publication
Journal Article
Langues
eng
Pagination
146-160Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001134
Pays : United Kingdom
Informations de copyright
Copyright: © 2021 Romila et al.
Déclaration de conflit d'intérêts
Conflict of Interest: The authors declare to have no conflicts of interest.
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