Streptococcus agalactiae do not penetrate human chorioamniotic membranes in vitro but alter their biomechanical properties.
Streptococcus agalactiae
chorioamniotic membranes
fetal membranes
group B streptococcus
perinatal infections
preterm prelabor rupture of membranes
tensile testing
Journal
Acta obstetricia et gynecologica Scandinavica
ISSN: 1600-0412
Titre abrégé: Acta Obstet Gynecol Scand
Pays: United States
ID NLM: 0370343
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
02
06
2021
received:
01
02
2021
accepted:
02
07
2021
pubmed:
14
7
2021
medline:
28
9
2021
entrez:
13
7
2021
Statut:
ppublish
Résumé
Vaginal colonization with Streptococcus agalactiae (group B streptococci) is hypothesized to constitute a risk factor for preterm prelabor rupture of membranes. In vitro studies have shown that S. agalactiae strains isolated from infants with neonatal sepsis adhere to chorion cells of the human chorioamniotic membrane. However, it is still unknown whether S. agalactiae strains penetrate the chorioamniotic membranes and whether S. agalactiae colonization affects the biomechanical properties of the membranes and thus contributes to increased risk of preterm prelabor rupture. The aim of this in vitro study was to explore if different strains of S. agalactiae penetrate and affect the biomechanical properties of human chorioamniotic membranes. Three different strains of S. agalactiae were obtained, one from an early-onset neonatal infection, one from a case of preterm prelabor rupture of membranes and one from a healthy pregnant carrier. Chorioamniotic membranes from elective cesarean deliveries were either incubated with S. agalactiae or mounted in a two-chamber incubation cell generating a "maternal" and a "fetal" chamber and incubated with S. agalactiae in the maternal chamber. Subsequently the membranes were examined to evaluate S. agalactiae attachment, penetration and the effect on the biomechanical properties. At 5 h after incubation, S. agalactiae adhered to the chorioamniotic membranes with increased number at 20 h. Streptococcus agalactiae did not penetrate the membranes even after 20 h of incubation. Streptococcus agalactiae increased the ultimate tensile stress needed to rupture the membranes and increased the work needed to rupture the membranes as well as the elastic modulus. Human chorioamniotic membranes constitute a physical barrier against S. agalactiae infections. Moreover, S. agalactiae infection leads to increased strength of the membranes.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1814-1821Subventions
Organisme : Novo Nordisk Foundation
ID : NNF16OC0020488
Informations de copyright
© 2021 Nordic Federation of Societies of Obstetrics and Gynecology (NFOG). Published by John Wiley & Sons Ltd.
Références
Bianchi-Jassir F, Seale AC, Kohli-Lynch M, et al. Preterm birth associated with group B streptococcus maternal colonization worldwide: systematic review and meta-analyses. Clin Infect Dis. 2017;65:S133-S142.
Matorras R, Garcia Perea A, Omenaca F, Usandizaga JA, Nieto A, Herruzo R. Group B streptococcus and premature rupture of membranes and preterm delivery. Gynecol Obstet Invest. 1989;27:14-18.
Zhang LX, Sun Y, Zhao H, et al. A Bayesian stepwise discriminant model for predicting risk factors of preterm premature rupture of membranes: a case-control study. Chin Med J (Engl). 2017;130:2416-2422.
Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med. 2000;342:1500-1507.
Cho CY, Tang YH, Chen YH, et al. Group B streptococcal infection in neonates and colonization in pregnant women: an epidemiological retrospective analysis. J Microbiol Immunol Infect. 2019;52:265-272.
Tzialla C, Berardi A, Farina C, et al. Strategies for preventing group B streptococcal infections in newborns: a nation-wide survey of Italian policies. Ital J Pediatr. 2017;43:98.
Keirse MJ, Ohlsson A, Treffers PE, Kanhani HHH. Prelabour rupture of the membranes preterm. In: Chalmers I, Enkin M, Keirse MJ, eds. Effective Care in Pregnancy and Childbirth. Oxford: Oxford University Press; 1989:666.
Steel JH, Malatos S, Kennea N, et al. Bacteria and inflammatory cells in fetal membranes do not always cause preterm labor. Pediatr Res. 2005;57:404-411.
Helmig R, Halaburt JT, Uldbjert N, Thomsen AC, Stenderup A. Increased cell adherence of group B streptococci from preterm infants with neonatal sepsis. Obstet Gynecol. 1990;76(5 Pt 1):825-827.
Strauss JF III. Extracellular matrix dynamics and fetal membrane rupture. Reprod Sci. 2013;20:140-153.
Hanberg P, Bue M, Birke Sorensen H, Soballe K, Tottrup M. Pharmacokinetics of single-dose cefuroxime in porcine intervertebral disc and vertebral cancellous bone determined by microdialysis. Spine J. 2016;16:432-438.
Tottrup M, Hardlei TF, Bendtsen M, et al. Pharmacokinetics of cefuroxime in porcine cortical and cancellous bone determined by microdialysis. Antimicrob Agents Chemother. 2014;58:3200-3205.
Persson KM, Forsgren A. Antimicrobial susceptibility of group B streptococci. Eur J Clin Microbiol. 1986;5:165-167.
Sørensen UB, Poulsen K, Ghezzo C, Margarit I, Kilian M. Emergence and global dissemination of host-specific Streptococcus agalactiae clones. mBio. 2010;1:e00178-e00210.
Login FH, Jensen HH, Pedersen GA, Amieva MR, Nejsum LN. The soluble extracellular domain of E-cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex. FASEB J. 2018:fj201800651. doi: https://doi.org/10.1096/fj.201800651. Epub ahead of print.
Pedersen GA, Jensen HH, Schelde AB, et al. The basolateral vesicle sorting machinery and basolateral proteins are recruited to the site of enteropathogenic E. coli microcolony growth at the apical membrane. PLoS One. 2017;12:e0179122.
Jensen HH, Pedersen HN, Stenkjaer E, Pedersen GA, Login FH, Nejsum LN. Tir is essential for the recruitment of Tks5 to enteropathogenic Escherichia coli pedestals. PLoS One. 2015;10:e0141871.
Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9:671-675.
Oxlund H, Helmig R, Halaburt JT, Uldbjerg N. Biomechanical analysis of human chorioamniotic membranes. Eur J Obstet Gynecol Reprod Biol. 1990;34:247-255.
Helmig R, Oxlund H, Petersen LK, Uldbjerg N. Different biomechanical properties of human fetal membranes obtained before and after delivery. Eur J Obstet Gynecol Reprod Biol. 1993;48:183-189.
Oxlund H, Andreassen TT. The roles of hyaluronic acid, collagen and elastin in the mechanical properties of connective tissues. J Anat. 1980;131:611-620.
Kjaergaard N, Helmig RB, Schonheyder HC, Uldbjerg N, Hansen ES, Madsen H. Chorioamniotic membranes constitute a competent barrier to group b streptococcus in vitro. Eur J Obstet Gynecol Reprod Biol. 1999;83:165-169.
Mirmonsef P, Gilbert D, Zariffard MR, et al. The effects of commensal bacteria on innate immune responses in the female genital tract. Am J Reprod Immunol. 2011;65:190-195.
Dusio GF, Cardani D, Zanobbio L, et al. Stimulation of TLRs by LMW-HA induces self-defense mechanisms in vaginal epithelium. Immunol Cell Biol. 2011;89:630-639.
Hein M, Petersen AC, Helmig RB, Uldbjerg N, Reinholdt J. Immunoglobulin levels and phagocytes in the cervical mucus plug at term of pregnancy. Acta Obstet Gynecol Scand. 2005;84:734-742.
Oyen ML, Calvin SE, Landers DV. Premature rupture of the fetal membranes: is the amnion the major determinant? Am J Obstet Gynecol. 2006;195:510-515.