Removing Disturbing Matrix Constituents from Biota Extracts from Total Extraction and Silicone-Based Passive Sampling.
Biota analysis
Cleanup
GC-HRMS analysis
Matrix effects
Multi-class hydrophobic organic chemicals
Silicone-based passive sampling
Journal
Environmental toxicology and chemistry
ISSN: 1552-8618
Titre abrégé: Environ Toxicol Chem
Pays: United States
ID NLM: 8308958
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
31
03
2021
received:
02
03
2021
accepted:
05
06
2021
pubmed:
14
7
2021
medline:
15
4
2022
entrez:
13
7
2021
Statut:
ppublish
Résumé
Contaminant analysis in biota extracts can be hampered by matrix interferences caused by, for example, co-extracted lipids that compromise the quality of the analytical data and require frequent maintenance of the analytical instruments. In the present study, using gas chromatography coupled to high resolution mass spectrometry (GC-HRMS), we aimed to develop and validate a straightforward, robust, and reproducible cleanup method with acceptable recoveries for diverse compound classes with a wide range of physicochemical properties representative of pollutant screening in biota extracts. We compared Oasis PRiME HLB cartridges, Agilent Captiva EMR-Lipid cartridges, and "Freeze-Out" with salmon lipids spiked with 113 target chemicals. The EMR-Lipid cartridges provided extracts with low matrix effects at reproducible recoveries of the multi-class target analytes (93 ± 9% and 95 ± 7% for low and high lipid amounts, respectively). The EMR-Lipid cartridges were further tested with spiked pork lipids submitted to total extraction or silicone-based passive sampling. Reproducible recoveries were achieved and matrix residuals were largely removed as demonstrated gravimetrically for both types of extracts. Ion suppression of halogenated compounds was not as efficiently removed by the cleanup of total and silicone-based extracts of pork lipids as for the salmon lipids. However, the samples with clean up provided better instrument robustness than those without cleanup. Hence, EMR-Lipid cartridges were shown to be efficient as a cleanup method in multi-class monitoring of biota samples and open up new possibilities as a suitable cleanup method for silicone extracts in biota passive sampling studies using GC-HRMS analysis. Environ Toxicol Chem 2021;40:2693-2704. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Substances chimiques
Lipids
0
Plant Extracts
0
Silicones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2693-2704Informations de copyright
© 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Références
Ahn YG, Seo J, Shin JH, Khim J, Hong J. 2006. Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration. Anal Chim Acta 576:31-36.
Allan IJ, Baek K, Haugen TO, Hawley KL, Høgfeldt AS, Lillicrap AD. 2013. In vivo passive sampling of nonpolar contaminants in brown trout (Salmo trutta). Environ Sci Technol 47:11660-11667.
Álvarez-Ruiz R, Picó Y. 2020. Analysis of emerging and related pollutants in aquatic biota. Trends Environ Anal Chem 25:e00082.
Anumol T, Lehotay SJ, Stevens J, Zweigenbaum J. 2017. Comparison of veterinary drug residue results in animal tissues by ultrahigh-performance liquid chromatography coupled to triple quadrupole or quadrupole-time-of-flight tandem mass spectrometry after different sample preparation methods, including use of a commercial lipid removal product. Anal Bioanal Chem 409:2639-2653.
Arce-López B, Lizarraga E, Flores-Flores M, Irigoyen Á, González-Peñas E. 2020. Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma. Talanta 206:120193.
Castilla-Fernández D, Moreno-González D, Beneito-Cambra M, Molina-Díaz A. 2019. Critical assessment of two sample treatment methods for multiresidue determination of veterinary drugs in milk by UHPLC-MS/MS. Anal Bioanal Chem 411:1433-1442.
Chen Z-Y, Ying S, Liu J-H, Zhan P-P, Zhao Y-G. 2016. PRiME pass-through cleanup for the fast determination of aflatoxins in human serum by using LC-MS/MS. Anal Methods 8:1457-1462.
Cruz R, Marques A, Casal S, Cunha SC. 2019. Fast and environmental-friendly methods for the determination of polybrominated diphenyl ethers and their metabolites in fish tissues and feed. Sci Total Environ 646:1503-1515.
Daniele G, Fieu M, Joachim S, James-Casas A, Andres S, Baudoin P, Bonnard M, Bonnard I, Geffard A, Vulliet E. 2016. Development of a multi-residue analysis of diclofenac and some transformation products in bivalves using QuEChERS extraction and liquid chromatography-tandem mass spectrometry. Application to samples from mesocosm studies. Talanta 155:1-7.
Desforges J-P, Eulaers I, Periard L, Sonne C, Dietz R, Letcher RJ. 2017. A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues. Chemosphere 176:243-248.
Gómez-Ariza JL, Bujalance M, Giráldez I, Velasco A, Morales E. 2002. Determination of polychlorinated biphenyls in biota samples using simultaneous pressurized liquid extraction and purification. J Chromatogr A 946:209-219.
Gómez-Ramos MM, Ucles S, Ferrer C, Fernández-Alba AR, Hernando MD. 2019. Exploration of environmental contaminants in honeybees using GC-TOF-MS and GC-Orbitrap-MS. Sci Total Environ 647:232-244.
Gonzalo-Lumbreras R, Sanz-Landaluze J, Cámara C. 2014. Analytical performance of two miniaturised extraction methods for triclosan and methyltriclosan, in fish roe and surimi samples. Food Chem 146:141-148.
Guo H, Wang H, Zheng J, Liu W, Zhong J, Zhao Q. 2018. Sensitive and rapid determination of glyphosate, glufosinate, bialaphos and metabolites by UPLC-MS/MS using a modified Quick Polar Pesticides Extraction method. Forensic Sci Int 283:111-117.
Hakme E, Lozano A, Uclés S, Gómez-Ramos MM, Fernández-Alba AR. 2018. High-throughput gas chromatography-mass spectrometry analysis of pesticide residues in spices by using the enhanced matrix removal-lipid and the sample dilution approach. J Chromatogr A 1573:28-41.
Hong J, Kim H-Y, Kim D-G, Seo J, Kim K-J. 2004. Rapid determination of chlorinated pesticides in fish by freezing-lipid filtration, solid-phase extraction and gas chromatography-mass spectrometry. J Chromatogr A 1038:27-35.
Jahnke A, Mayer P, Broman D, McLachlan MS. 2009. Possibilities and limitations of equilibrium sampling using polydimethylsiloxane in fish tissue. Chemosphere 77:764-770.
Jahnke A, McLachlan MS, Mayer P. 2008. Equilibrium sampling: Partitioning of organochlorine compounds from lipids into polydimethylsiloxane. Chemosphere 73:1575-1581.
Jensen S, Häggberg L, Jörundsdóttir H, Odham G. 2003. A quantitative lipid extraction method for residue analysis of fish involving nonhalogenated solvents. J Agric Food Chem 51:5607-5611.
Jin L, Escher BI, Limpus CJ, Gaus C. 2015. Coupling passive sampling with in vitro bioassays and chemical analysis to understand combined effects of bioaccumulative chemicals in blood of marine turtles. Chemosphere 138:292-299.
Krätschmer K, Cojocariu C, Schächtele A, Malisch R, Vetter W. 2018. Chlorinated paraffin analysis by gas chromatography Orbitrap high-resolution mass spectrometry: Method performance, investigation of possible interferences and analysis of fish samples. J Chromatogr A 1539:53-61.
Lozano A, Uclés S, Uclés A, Ferrer C, Fernández-Alba AR. 2018. Pesticide residue analysis in fruit- and vegetable-based baby foods using GC-Orbitrap MS. J AOAC Int 101:374-382.
Lu Z, Deng F, He R, Tan L, Luo X, Pan X, Yang Z. 2019. A pass-through solid-phase extraction clean-up method for the determination of 11 quinolone antibiotics in chicken meat and egg samples using ultra-performance liquid chromatography tandem mass spectrometry. Microchem J 151:104213.
Lucas D, Zhao L. 2015. PAH analysis in salmon with enhanced matrix removal. Application Note 5991-6088EN. Agilent Technologies, Wilmington, DE, USA.
Ma Y, Cui K, Zeng F, Wen J, Liu H, Zhu F, Ouyang G, Luan T, Zeng Z. 2013. Microwave-assisted extraction combined with gel permeation chromatography and silica gel cleanup followed by gas chromatography-mass spectrometry for the determination of organophosphorus flame retardants and plasticizers in biological samples. Anal Chim Acta 786:47-53.
Moreno-González D, Hamed AM, Gilbert-López B, Gámiz-Gracia L, García-Campaña AM. 2017. Evaluation of a multiresidue capillary electrophoresis-quadrupole-time-of-flight mass spectrometry method for the determination of antibiotics in milk samples. J Chromatogr A 1510:100-107.
Muz M, Escher BI, Jahnke A. 2020. Bioavailable environmental pollutant patterns in sediments from passive equilibrium sampling. Environ Sci Technol 54(24):15861-15871.
Nagornov KO, Zennegg M, Kozhinov AN, Tsybin YO, Bleiner D. 2020. Trace-level persistent organic pollutant analysis with gas-chromatography Orbitrap mass spectrometry-Enhanced performance by complementary acquisition and processing of time-domain data. J Am Soc Mass Spectrom 31:257-266.
Niu L, Carmona E, König M, Krauss M, Muz M, Xu C, Zou D, Escher BI. 2020. Mixture risk drivers in freshwater sediments and their bioavailability determined using passive equilibrium sampling. Environ Sci Technol 54(20):13197-13206.
Peterson AC, Hauschild J-P, Quarmby ST, Krumwiede D, Lange O, Lemke RAS, Grosse-Coosmann F, Horning S, Donohue TJ, Westphall MS, Coon JJ, Griep-Raming J. 2014. Development of a GC/Quadrupole-Orbitrap Mass Spectrometer. Part I: Design and characterization. Anal Chem 86:10036-10043.
Pourchet M, Narduzzi L, Jean A, Guiffard I, Bichon E, Cariou R, Guitton Y, Hutinet S, Vlaanderen J, Meijer J, Le Bizec B, Antignac J-P. 2021. Non-targeted screening methodology to characterise human internal chemical exposure: Application to halogenated compounds in human milk. Talanta 225:121979.
Reiter E. 2017. Combination of silicone-based sampling with bioanalytical characterization of the mixture toxicity of environmental pollutants in mammals (in German). MSc thesis. Leipzig University, Leipzig, Germany.
Rojo-Nieto E, Muz M, Koschorreck J, Rüdel H, Jahnke A. 2019. Passive equilibrium sampling of hydrophobic organic compounds in homogenised fish tissues of low lipid content. Chemosphere 220:501-504.
Rusina TP, Carlsson P, Vrana B, Smedes F. 2017. Equilibrium passive sampling of POP in lipid-rich and lean fish tissue: Quality control using performance reference compounds. Environ Sci Technol 51:11250-11257.
Rusina TP, Smedes F, Klanova J, Booij K, Holoubek I. 2007. Polymer selection for passive sampling: A comparison of critical properties. Chemosphere 68:1344-1351.
Sapozhnikova Y, Lehotay SJ. 2013. Multi-class, multi-residue analysis of pesticides, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, polybrominated diphenyl ethers and novel flame retardants in fish using fast, low-pressure gas chromatography-tandem mass spectrometry. Anal Chim Acta 758:80-92.
Shahpoury P, Hageman KJ. 2013. Pressurized liquid extraction of polycyclic aromatic hydrocarbons from silicone rubber passive samplers. J Chromatogr A 1314:1-6.
Shin JH, Boo HO, Bang E, Gorinstein S, Seo J. 2012. Development of a cleanup method for polybrominated diphenyl ether (PBDE) in fish by freezing-lipid filtration. Eur Food Res Technol 235:295-301.
Simon E, Lamoree MH, Hamers T, Weiss JM, Balaam J, De Boer J, Leonards PEG. 2010. Testing endocrine disruption in biota samples: A method to remove interfering lipids and natural hormones. Environ Sci Technol 44:8322-8329.
Stubleski J, Kukucka P, Salihovic S, Lind PM, Lind L, Kärrman A. 2018. A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction. J Chromatogr A 1546:18-27.
US Environmental Protection Agency. 2000. EPI suite. KOWWIN, Ver 1.67. “Freeware” provided by USEPA. One of eleven separate programs that provide resulting estimation information, KOWWIN estimates octanol-water partition coefficient. Washington, DC.
US Environmental Protection Agency. 2011. Title 40: Protection of Environment. Part 136-Guidelines establishing test procedures for the analysis of pollutants. Appendix B to Part 136-Definition and procedure for the determination of the method detection limit-Revision 1.11.
Wickrama-Arachchige AU-K, Hirabayashi T, Imai Y, Guruge KS, Dharmaratne TS, Ohura T. 2020. Accumulation of halogenated polycyclic aromatic hydrocarbons by different tuna species, determined by high-resolution gas chromatography Orbitrap mass spectrometry. Environ Pollut 256:113487.
Zhang X, Danaceau JP, Chambers EE. 2015. Improvements in recovery, reproducibility, and matrix effects with Oasis PRiME HLB, a novel solid phase extraction sorbent. Application Note 720005495EN. Waters, Milford, MA, USA.
Zhang Y, Zhao Y-G, Cheng H-L, Muhammad N, Chen W-S, Zeng X-Q, Zhu Y. 2018. Fast determination of fipronil and its metabolites in seafood using PRiME pass-through cleanup followed by isotope dilution UHPLC-MS/MS. Anal Methods 10:1673-1679.
Zhao L, Lucas D. 2015. Multiresidue analysis of veterinary drugs in bovine liver by LC/MS/MS. Application Note 5991-6096EN. Agilent Technologies, Wilmington, DE, USA.
Zhao L, Lucas D. 2017. Efficiency of Biological Fluid Matrix Removal Using Agilent Captiva EMR-Lipid Cleanup. Application Note 5991-8006EN. Agilent Technologies, Wilmington, DE, USA.
Zhao L, Lucas D, Long D, Richter B, Stevens J. 2018. Multi-class multi-residue analysis of veterinary drugs in meat using enhanced matrix removal lipid cleanup and liquid chromatography-tandem mass spectrometry. J Chromatogr A 1549:14-24.
Zhao L, Szakas T, Churley M, Lucas D. 2019. Multi-class multi-residue analysis of pesticides in edible oils by gas chromatography-tandem mass spectrometry using liquid-liquid extraction and enhanced matrix removal lipid cartridge cleanup. J Chromatogr A 1584:1-12.