The dorsal blastopore lip is a source of signals inducing planar cell polarity in the Xenopus neural plate.
Xenopus embryos
Dorsal blastopore lip
Induction
Neuroectoderm
PCP
Prickle3
Signaling
Vangl2
Journal
Biology open
ISSN: 2046-6390
Titre abrégé: Biol Open
Pays: England
ID NLM: 101578018
Informations de publication
Date de publication:
15 07 2021
15 07 2021
Historique:
received:
12
04
2021
accepted:
04
06
2021
pubmed:
15
7
2021
medline:
17
3
2022
entrez:
14
7
2021
Statut:
ppublish
Résumé
Coordinated polarization of cells in the tissue plane, known as planar cell polarity (PCP), is associated with a signaling pathway critical for the control of morphogenetic processes. Although the segregation of PCP components to opposite cell borders is believed to play a critical role in this pathway, whether PCP derives from egg polarity or preexistent long-range gradient, or forms in response to a localized cue, remains a challenging question. Here we investigate the Xenopus neural plate, a tissue that has been previously shown to exhibit PCP. By imaging Vangl2 and Prickle3, we show that PCP is progressively acquired in the neural plate and requires a signal from the posterior region of the embryo. Tissue transplantations indicated that PCP is triggered in the neural plate by a planar cue from the dorsal blastopore lip. The PCP cue did not depend on the orientation of the graft and was distinct from neural inducers. These observations suggest that neuroectodermal PCP is not instructed by a preexisting molecular gradient but induced by a signal from the dorsal blastopore lip.
Identifiants
pubmed: 34259326
pii: 270845
doi: 10.1242/bio.058761
pmc: PMC8325942
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS100759
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122492
Pays : United States
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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