Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.

Amino Acid Motifs Amino Acid Sequence Cell Cycle Proteins / metabolism Chromosome Segregation Feedback, Physiological HEK293 Cells HeLa Cells Humans Kinetochores / metabolism Mitosis Phosphoprotein Phosphatases / metabolism Phosphorylation Phosphoserine / metabolism Protein Binding Protein Serine-Threonine Kinases / metabolism Proteins / chemistry Proto-Oncogene Proteins / metabolism Polo-Like Kinase 1
Apolo1 PLK1 PP1γ chromosome segregation kinetochore mitosis

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
13 07 2021
Historique:
received: 22 09 2019
revised: 01 04 2020
accepted: 15 06 2021
entrez: 14 7 2021
pubmed: 15 7 2021
medline: 9 2 2022
Statut: ppublish

Résumé

Stable transmission of genetic material during cell division requires accurate chromosome segregation. PLK1 dynamics at kinetochores control establishment of correct kinetochore-microtubule attachments and subsequent silencing of the spindle checkpoint. However, the regulatory mechanism responsible for PLK1 activity in prometaphase has not yet been affirmatively identified. Here we identify Apolo1, which tunes PLK1 activity for accurate kinetochore-microtubule attachments. Apolo1 localizes to kinetochores during early mitosis, and suppression of Apolo1 results in misaligned chromosomes. Using the fluorescence resonance energy transfer (FRET)-based PLK1 activity reporter, we found that Apolo1 sustains PLK1 kinase activity at kinetochores for accurate attachment during prometaphase. Apolo1 is a cognate substrate of PLK1, and the phosphorylation enables PP1γ to inactivate PLK1 by dephosphorylation. Mechanistically, Apolo1 constitutes a bridge between kinase and phosphatase, which governs PLK1 activity in prometaphase. These findings define a previously uncharacterized feedback loop by which Apolo1 provides fine-tuning for PLK1 to guide chromosome segregation in mitosis.

Identifiants

DOI: 10.1016/j.celrep.2021.109343 PMID: 34260926 PMC: PMC8358895
pubmed: 34260926
pii: S2211-1247(21)00719-1
doi: 10.1016/j.celrep.2021.109343
pmc: PMC8358895
mid: NIHMS1724377
pii:
doi:

Substances chimiques

Cell Cycle Proteins 0
Proteins 0
Proto-Oncogene Proteins 0
Phosphoserine 17885-08-4
Protein Serine-Threonine Kinases EC 2.7.11.1
Phosphoprotein Phosphatases EC 3.1.3.16

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109343

Subventions

Organisme : NCI NIH HHS
ID : R01 CA164133
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK056292
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK115812
Pays : United States

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Leilei Xu (L)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.

Mahboob Ali (M)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.

Wenxiu Duan (W)

Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.

Xiao Yuan (X)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address: yuanxiao@ustc.edu.cn.

Fatima Garba (F)

Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.

McKay Mullen (M)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.

Binwen Sun (B)

National Chromatographic Research and Analysis Center, Dalian 116023, China.

Ina Poser (I)

Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Hequan Duan (H)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA; Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.

Jianlin Lu (J)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.

Ruijun Tian (R)

Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.

Yushu Ge (Y)

Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.

Lingluo Chu (L)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.

Weijun Pan (W)

Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.

Dongmei Wang (D)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.

Anthony Hyman (A)

Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Hadiyah Green (H)

Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.

Lin Li (L)

Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.

Zhen Dou (Z)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China. Electronic address: douzhen@ustc.edu.cn.

Dan Liu (D)

Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China. Electronic address: dliu919@ustc.edu.cn.

Xing Liu (X)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA. Electronic address: xing1017@ustc.edu.cn.

Xuebiao Yao (X)

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA. Electronic address: yaoxb@ustc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Motifs d'acides aminés › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cycle cellulaire › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Ségrégation des chromosomes › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Rétrocontrôle physiologique › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Centromère › Kinétochores › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Mitose › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphoprotein Phosphatases › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Métabolisme › Phosphorylation › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides phosphoaminés › Phosphosérine › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Feedback control of PLK1 by Apolo1 ensures...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Feedback control of PLK1 by Apolo1 ensures...