A mask-based diagnostic platform for point-of-care screening of Covid-19.
Aptamers
Detection
Electrochemistry
Exhaled breath condensate (EBC)
Real-time reverse-transcription polymerase chain reaction (RT-PCR)
SARS-CoV-2
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Nov 2021
15 Nov 2021
Historique:
received:
20
06
2021
accepted:
02
07
2021
pubmed:
15
7
2021
medline:
22
9
2021
entrez:
14
7
2021
Statut:
ppublish
Résumé
Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200-500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL
Identifiants
pubmed: 34260968
pii: S0956-5663(21)00523-6
doi: 10.1016/j.bios.2021.113486
pmc: PMC8264268
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113486Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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