A novel 2 bp deletion variant in Ovine-DRB1 gene is associated with increased Visna/maedi susceptibility in Turkish sheep.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 07 2021
14 07 2021
Historique:
received:
02
04
2021
accepted:
01
07
2021
entrez:
15
7
2021
pubmed:
16
7
2021
medline:
6
11
2021
Statut:
epublish
Résumé
Visna/maedi (VM) is a multisystemic lentivirus infection of sheep that affecting sheep industry across the globe. TMEM154 gene has been identified to be a major VM-associated host gene, nevertheless, a recent study showed that the frequency of the VM-resistant TMEM154 haplotypes was very low or absent in indigenous sheep. Thus, the present study was designed to determine other possible co-receptors associated with VM. For this purpose, DRB1 gene, which is renowned for its role in host immune response against various diseases was targeted. A total number of 151 case-control matched pairs were constructed from 2266 serologically tested sheep. A broad range of DRB1 haplotype diversity was detected by sequence-based genotyping. Moreover, a novel 2 bp deletion (del) in the DRB1 intron 1 was identified. For the final statistic, the sheep carrying VM-resistant TMEM154 diplotypes were removed and a McNemar's test with a matched pairs experimental design was conducted. Consequently, it was identified for the first time that the 2 bp del variant is a genetic risk factor for VM (p value 0.002; chi-square 8.31; odds ratio 2.9; statistical power 0.90) in the dominant model. Thus, negative selection for 2 bp del variant could decrease VM infection risk in Turkish sheep.
Identifiants
pubmed: 34262107
doi: 10.1038/s41598-021-93864-8
pii: 10.1038/s41598-021-93864-8
pmc: PMC8280167
doi:
Substances chimiques
Membrane Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14435Informations de copyright
© 2021. The Author(s).
Références
Vet J. 2009 Oct;182(1):31-7
pubmed: 18755622
Vet Res. 1998 May-Aug;29(3-4):341-67
pubmed: 9689746
Vet J. 2013 Sep;197(3):607-12
pubmed: 23643869
Adv Bioinformatics. 2014;2014:402492
pubmed: 25147564
Rev Sci Tech. 2017 Dec;36(3):899-903
pubmed: 30160692
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Immunogenetics. 2008 Dec;60(12):749-58
pubmed: 18797863
J Parasitol Res. 2014;2014:272601
pubmed: 24782918
Parasitology. 2005 Sep;131(Pt 3):403-9
pubmed: 16178362
Behav Res Methods. 2009 Nov;41(4):1149-60
pubmed: 19897823
Viruses. 2018 Dec 08;10(12):
pubmed: 30544780
Cell Mol Life Sci. 2019 Sep;76(18):3525-3542
pubmed: 31101936
J Anim Sci. 2013 Nov;91(11):5114-21
pubmed: 23989875
Anim Genet. 2014 Apr;45(2):297-300
pubmed: 24303974
Vet Microbiol. 1984 Sep;9(5):445-51
pubmed: 6093339
PLoS Genet. 2012 Jan;8(1):e1002467
pubmed: 22291605
Nat Protoc. 2020 May;15(5):1829-1852
pubmed: 32269383
J Proteome Res. 2020 Jun 5;19(6):2304-2315
pubmed: 32308001
J Immunol. 1994 Aug 15;153(4):1665-73
pubmed: 7519208
Immunogenetics. 2010 Jan;62(1):31-9
pubmed: 19943043
Vet J. 2001 Mar;161(2):208-10
pubmed: 11243691
J Vet Med Sci. 2014 Mar 1;76(2):211-8
pubmed: 24141278
PLoS One. 2013 May 15;8(5):e63901
pubmed: 23691111
Arch Virol. 2013 Mar;158(3):559-70
pubmed: 23124887
Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13258-63
pubmed: 20622157
PLoS One. 2012;7(10):e47829
pubmed: 23082221
N Z Vet J. 2006 Aug;54(4):153-60
pubmed: 16915336
PLoS One. 2015 Mar 23;10(3):e0120890
pubmed: 25799151
Psychometrika. 1947 Jun;12(2):153-7
pubmed: 20254758
Bioinformatics. 2005 Jan 15;21(2):263-5
pubmed: 15297300
J Virol. 2004 Jul;78(14):7518-22
pubmed: 15220425
Anim Genet. 2009 Oct;40(5):583-9
pubmed: 19397512
Science. 2009 Apr 24;324(5926):532-6
pubmed: 19390051
Nat Genet. 2014 Mar;46(3):234-44
pubmed: 24509480
J Immunol. 1994 Aug 1;153(3):1141-9
pubmed: 8027545
Vet Res. 2004 May-Jun;35(3):257-74
pubmed: 15210075
Immunogenetics. 2010 Feb;62(2):75-83
pubmed: 20049428
Animals (Basel). 2019 Sep 15;9(9):
pubmed: 31540148
J Anim Sci. 2015 Jan;93(1):41-5
pubmed: 25568355
Vet Med (Auckl). 2018 May 21;9:11-21
pubmed: 30050863
Vet Res. 2018 Apr 19;49(1):36
pubmed: 29673399
Can Vet J. 2017 Feb;58(2):183-186
pubmed: 28216689
Prev Vet Med. 1997 May;30(2):155-69
pubmed: 9234419
Am J Hum Genet. 2003 Nov;73(5):1162-9
pubmed: 14574645