The chromosome-scale assembly of the Canary Islands endemic spider Dysdera silvatica (Arachnida, Araneae) sheds light on the origin and genome structure of chemoreceptor gene families in chelicerates.
Araneae
Dysdera
Hi-C
chemoreceptors
chromosome-level assembly
gene clusters
gene family
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
21
06
2021
received:
12
04
2021
accepted:
12
07
2021
pubmed:
17
7
2021
medline:
22
12
2021
entrez:
16
7
2021
Statut:
ppublish
Résumé
Here, we present the chromosome-level genome assembly of Dysdera silvatica Schmidt, 1981, a nocturnal ground-dwelling spider endemic from the Canary Islands. The genus Dysdera has undergone a remarkable diversification in this archipelago mostly associated with shifts in the level of trophic specialization, becoming an excellent model to study the genomic drivers of adaptive radiations. The new assembly (1.37 Gb; scaffold N50 of 174.2 Mb), was performed using the chromosome conformation capture scaffolding technique, represents a continuity improvement of more than 4500 times with respect to the previous version. The seven largest scaffolds or pseudochromosomes, which cover 87% of the total assembly size, probably correspond with the seven chromosomes of the karyotype of this species, including a characteristic large X chromosome. To illustrate the value of this new resource we performed a comprehensive analysis of the two major arthropod chemoreceptor gene families (i.e., gustatory and ionotropic receptors). We identified 545 chemoreceptor sequences distributed across all pseudochromosomes, with a notable underrepresentation in the X chromosome. At least 54% of them localize in 83 genomic clusters with a significantly lower evolutionary distances between them than the average of the family, suggesting a recent origin of many of them. This chromosome-level assembly is the first high-quality genome representative of the Synspermiata clade, and just the third among spiders, representing a new valuable resource to gain insights into the structure and organization of chelicerate genomes, including the role that structural variants, repetitive elements and large gene families played in the extraordinary biology of spiders.
Identifiants
pubmed: 34268885
doi: 10.1111/1755-0998.13471
doi:
Banques de données
RefSeq
['QLNU00000000', 'PRJNA475203', 'QLNU02000000', 'SRR13907633', 'SRR13907634']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-390Subventions
Organisme : Ministerio de Economía y Competitividad of Spain
ID : CGL2016-75255
Organisme : Ministerio de Economía y Competitividad of Spain
ID : CGL2016-80651
Organisme : Ministerio de Economía y Competitividad of Spain
ID : PID2019-103947GB
Organisme : Ministerio de Economía y Competitividad of Spain
ID : PID2019-105794GB
Organisme : Ministerio de Economía y Competitividad of Spain
ID : BES-2017-081740
Organisme : Comissió Interdepartamental de Recerca I Innovació Tecnològica of Catalonia, Spain
ID : 2017SGR83
Organisme : Comissió Interdepartamental de Recerca I Innovació Tecnològica of Catalonia, Spain
ID : 2017SGR1287
Informations de copyright
© 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
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