Investigation of toxicity attenuation mechanism of tetrahydroxy stilbene glucoside in Polygonum multiflorum Thunb. by Ganoderma lucidum.
Biological detoxification
Ganoderma lucidum
Idiosyncratic hepatotoxicity
Lignin peroxidase
Polygonum multiflorum Thunb.
Tetrahydroxystilbene glucoside
Journal
Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310
Informations de publication
Date de publication:
15 Nov 2021
15 Nov 2021
Historique:
received:
13
11
2020
revised:
28
06
2021
accepted:
12
07
2021
pubmed:
17
7
2021
medline:
12
1
2022
entrez:
16
7
2021
Statut:
ppublish
Résumé
The idiosyncratic hepatotoxicity of Polygonum multiflorum Thunb. (PM) has attracted great interest, and tetrahydroxy stilbene glucoside (TSG) was the main idiosyncratic hepatotoxicity constituent, but biological detoxification on idiosyncratic hepatotoxicity of PM was not well investigated. This study aimed to illustrate biological detoxification mechanism on PM-induced idiosyncratic hepatotoxicity by Ganoderma lucidum (G. lucidum). G. lucidum was used for biological detoxification of tetrahydroxy stilbene glucoside (TSG)-induced idiosyncratic hepatotoxicity of PM. The TSG consumption and products formation were dynamically determined during transformation using high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-MSn). The transformation invertases (β-D-glucosidase and lignin peroxidase) were evaluated by using intracellular and extracellular distribution and activity assay. The key functions of lignin peroxidase (LiP) were studied by experiments of adding inhibitors and agonists. The entire TSG transformation process was confirmed in vitro simulated test. The cellular toxicity of TSG and the transformation products was detected by MTT. A suitable biotransformation system of TSG was established with G. lucidum, then p-hydroxybenzaldehyde and 2,3,5-trihydroxybenzaldehyde can be found as transformation products of TSG. The transformation mechanism involves two extracellular enzymes, β-D-glucosidase and LiP. β-D-glucosidase can remove glycosylation of TSG firstly and then LiP can break the double bond of remaining glycosides. The toxicity of TSG after biotransformation by G. lucidum was attenuated. This study would reveal a novel biological detoxification method for PM and explain degradation processes of TSG by enzymic methods.
Identifiants
pubmed: 34271114
pii: S0378-8741(21)00650-4
doi: 10.1016/j.jep.2021.114421
pii:
doi:
Substances chimiques
2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside
0
Glucosides
0
Stilbenes
0
Peroxidases
EC 1.11.1.-
lignin peroxidase
EC 1.11.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114421Informations de copyright
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