Analysis of miRNA expression profile in lung tissues of an intermittent hypoxia rat model.

Animals Disease Models, Animal Hypoxia / metabolism Lung / metabolism MicroRNAs / metabolism Rats Signal Transduction / physiology Sleep Apnea, Obstructive / metabolism
Intermittent hypoxia Obstructive sleep apnea Pathway Target gene microRNA

Journal

Respiratory physiology & neurobiology
ISSN: 1878-1519
Titre abrégé: Respir Physiol Neurobiol
Pays: Netherlands
ID NLM: 101140022

Informations de publication

Date de publication:
12 2021
Historique:
received: 02 04 2021
revised: 21 06 2021
accepted: 07 07 2021
pubmed: 18 7 2021
medline: 2 2 2022
entrez: 17 7 2021
Statut: ppublish

Résumé

We screened key miRNAs in an intermittent hypoxia rat model and explored the biological roles of downstream target genes and related regulatory pathways. We analyzed the expression profile of miRNAs in the lung tissues of rats in the 5 % (IH1), 7.5 % (IH2), 10 % (IH3), 12.5 % (IH4) oxygen concentration and negative control (NC) groups and identified common miRNAs. Multiple differentially expressed miRNAs were detected, and intersection of their expression profiles yielded 10 common miRNAs with 929 target genes mainly distributed in the nucleus. Molecular functions pertained mainly to the activation of transcription factors, while biological processes focused on cell interaction and signal transduction. Among signaling pathways, the top 5 included the LKB1 signaling, nectin adhesion, and S1P pathways. 8 of 10 common miRNAs had excellent diagnostic value for detecting intermittent hypoxia. The miRNAs binds to the target gene might play a key role in the pathophysiological process of OSA through the LKB1/AMPK and S1P/Akt/eNOS signaling pathways.

Identifiants

DOI: 10.1016/j.resp.2021.103741 PMID: 34273552
pubmed: 34273552
pii: S1569-9048(21)00126-9
doi: 10.1016/j.resp.2021.103741
pii:
doi:

Substances chimiques

MicroRNAs 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103741

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Zhijuan Liu (Z)

Kunming Medical University, Kunming, Yunnan, 650500, China.

Li Ai (L)

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

Ran Li (R)

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

Yuan Yang (Y)

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

Keli Chen (K)

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

Chunxia He (C)

Kunming Medical University, Kunming, Yunnan, 650500, China.

Yongxia Li (Y)

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China. Electronic address: liyongxia0720@126.com.

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Classifications MeSH

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